A pilot study to characterize the effects of ageing on the innate immune response against influenza
Completed
- Conditions
- fluInfluenza10047438
- Registration Number
- NL-OMON31944
- Lead Sponsor
- ederlands Vaccin Instituut
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 94
Inclusion Criteria
Subjects have to fulfil all the following criteria:
- 20-40 years or >= 65 years old
- vaccinated with most recent seasonal influenza vaccine
- having a signed informed consent form
Exclusion Criteria
The exclusion criteria are:
- immune deficiencies
- haematological disorders
- bleeding disorders
- usage of anticoagulants, corticosteroids, NSAIDs and/or statins
- diabetes mellitus
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>To determine functional immune responses, after in vitro influenza infection of<br /><br>dendritic cells isolated from young adults and elderly people. The responses<br /><br>will be characterized based on; influenza-specific mRNA expressiom, maturation<br /><br>molecule expression, inhibitory molecule expression, cytokine production and T<br /><br>cell stimulating capacity.</p><br>
- Secondary Outcome Measures
Name Time Method <p>To determine the variation of the assays for qualifying the innate immune<br /><br>response of DC isolated from young adults or elderly individuals.</p><br>