The Effect of Environmental Factors on the Macro-genome and Metabolism of Obese Patients.
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 发起方
- 入组人数
- 300
- 试验地点
- 1
- 主要终点
- BMI
研究概览
简要总结
The present study was designed to include obese patients diagnosed with obesity in the First Affiliated Hospital of Chongqing Medical University and a healthy control population. By analysing the relationship between metabolic parameters and macrogenomes in obesity, identifying key metabolic and macrogenomic differences between obese patients and healthy populations, and establishing the association between environmental factors and obesity-related metabolic disorders, the investigators will be able to provide new biomarkers and intervention targets for obesity prevention and treatment.
详细描述
Obese patients who attended the First Hospital of Chongqing Medical University and intended to undergo bariatric surgery were screened according to the inclusion and exclusion criteria. Patients' basic personal information and biological specimens including blood, faeces and urine were collected for metabolic and macro-genomic analyses. Air pollution exposure of the study subjects was collected by telephone follow-up. Metabolite profiles in blood and urine were analysed using liquid chromatography-mass spectrometry (LC-MS). Microbial community composition in faecal samples was analysed using 16S rRNA gene sequencing. Metabolic and macrogenomic differences between obese and control groups were compared using t-test or Mann-Whitney U-test. Logistic regression analyses were used to assess the independent effects of different environmental factors on metabolism and macrogenome. Identify key metabolites and microorganisms associated with obesity using Random Forest and Principal Component Analysis.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age ≥18 years;
- •Obese patients with BMI ≥30kg/m2;
- •Patients did not undergo surgical treatment before enrolment;
- •Willing to participate in this study and sign the informed consent form;
- •Complete clinical data.
排除标准
- •Patients with systemic diseases such as severe cardiorespiratory insufficiency that affect the choice of treatment regimen;
- •Pregnant or lactating women;
- •Patients who are not suitable for enrolment as assessed by the investigator;
- •Patients with incomplete clinical data.
结局指标
主要结局
BMI
时间窗: BMI was examed when first join the study, whith through the whole study period, an average of 1 month.
BMI = weight / height squared (kg/m2)
次要结局
未报告次要终点
研究者
Dong Peng
Principal Investigator
First Affiliated Hospital of Chongqing Medical University