MedPath

A Trial to Compare Two Methods of Obtaining Tissue for Diagnosis of Sarcoidosis Namely the Conventional Method or the Ultrasound Guided Method

Not Applicable
Conditions
Sarcoidosis
Interventions
Other: cTBNA without ROSE
Other: EBUS-TBNA without ROSE
Other: cTBNA with ROSE
Other: EBUS-TBNA with ROSE
Registration Number
NCT02472808
Lead Sponsor
All India Institute of Medical Sciences, New Delhi
Brief Summary

This trial compares the yield of cTBNA (conventional Transbronchial Needle Aspiration) versus the EBUS-TBNA (Endobronchial Ultrasound guided TBNA) for obtaining cytology when they are combined with endobronchial biopsy and transbronchial lung biopsy and rapid onsite examination (ROSE) of the obtained smears in the diagnosis of suspected sarcoidosis patients visiting our hospital for evaluation of mediastinal lymphadenopathy.

Detailed Description

This study is investigator initiated, prospective, unblinded randomized study comparing conventional TBNA with EBUS-TBNA for obtaining tissue samples from mediastinal lymph nodes for the diagnosis of sarcoidosis when combined with transbronchial lung biopsy and endobronchial biopsy. It will be conducted on 80 consecutive patients with suspected sarcoidosis presenting to Department of Pulmonary Medicine and Sleep Disorders in AIIMS (All India Institute of Medical Sciences), New Delhi. This study will help to know whether EBUS (Endobronchial Ultrasound guided) or conventional TBNA is better for obtaining tissue samples from mediastinal lymph nodes for the diagnosis of sarcoidosis. It will also help to know the value of adding rapid onsite evaluation of the cytology smears to both the procedures. It will also help to determine the overall yield of different procedures when they are combined for obtaining tissue samples for a diagnosis of sarcoidosis.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
80
Inclusion Criteria
  • Age > 18 years and suspected Stage 1 (mediastinal or hilar Lymphadenopathy (LAP)) or Stage 2 ( LAP and parenchymal abnormalities) sarcoidosis
  • Significant mediastinal LAP (Short Axis Diameter more than 10mm)
  • Lymph Node (LN) enlargement in station 4, 7, 10 or 11 and CT chest findings consistent with sarcoidosis.
  • With or without supportive evidence of sarcoidosis - hypercalcemia, calciuria, raised ACE levels or restriction/obstruction on Pulmonary Function Tests (PFT).
Exclusion Criteria
  • Obvious other organ involvement with possibility to confirm granuloma with minimally invasive diagnostic procedure (Skin biopsy, superficial lymph nodes, Lofgren's Syndrome)
  • Sarcoidosis Stage 3 and 4
  • Mediastinal Nodes less than 10 mm in short axis diameter
  • Absence of right paratracheal and subcarinal Lymph node enlargement more than 1 cm in short axis diameter
  • Those who received empiric Steroid for >2 weeks in preceding 3 months
  • Contraindication to bronchoscopy and TBNA (Coagulopathy, Hypoxemia)
  • Unwilling to give consent

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
cTBNA without ROSEcTBNA without ROSEPatients who will undergo conventional TBNA without ROSE + Endobronchial biopsy + Transbronchial Lung Biopsy
EBUS-TBNA without ROSEEBUS-TBNA without ROSEPatients who will undergo EBUS-TBNA without ROSE + Endobronchial biopsy + Transbronchial Lung Biopsy
cTBNA with ROSEcTBNA with ROSEPatients who will undergo conventional TBNA with ROSE + Endobronchial biopsy + Transbronchial Lung Biopsy
EBUS-TBNA with ROSEEBUS-TBNA with ROSEPatients who will undergo EBUS-TBNA with ROSE + Endobronchial biopsy + Transbronchial Lung Biopsy
Primary Outcome Measures
NameTimeMethod
Diagnostic Yield of Conventional TBNA vs EBUS TBNA with or without ROSE6 months

Diagnostic Yield is defined as the number of patients with presence of non caseating granuloma on cytology smear divided by total number of sarcoidosis patient expressed as percentage

Secondary Outcome Measures
NameTimeMethod
Sensitivity of conventional TBNA to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months
Sensitivity of conventional TBNA when combined with ROSE to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months
Specificity of conventional TBNA when combined with ROSE to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months
Specificity of EBUS-TBNA to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months
Negative predictive value of EBUS-TBNA in the diagnosis of Sarcoidosis6 months
Yield of EBUS-TBNA in Conventional TBNA with or without ROSE negative patients6 months

Yield is defined as the number of patients with presence of non caseating granuloma on cytology smear divided by total number of sarcoidosis patient expressed as percentage

Specificity of conventional TBNA to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months
Specificity of EBUS-TBNA when combined with ROSE to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months
Positive predictive value of EBUS-TBNA to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months
Negative predictive value of conventional TBNA in the diagnosis of Sarcoidosis6 months
Negative predictive value of conventional TBNA when combined with ROSE in the diagnosis of Sarcoidosis6 months
Positive predictive value of conventional TBNA when combined with ROSE to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months
Negative predictive value of EBUS-TBNA when combined with ROSE in the diagnosis of Sarcoidosis6 months
Positive predictive value of conventional TBNA to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months
Positive predictive value of EBUS-TBNA when combined with ROSE to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months
Sensitivity of EBUS-TBNA to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months
Sensitivity of EBUS-TBNA when combined with ROSE to detect Non Caseating Granuloma for the diagnosis of Sarcoidosis6 months

Trial Locations

Locations (1)

All India Institute of Medical Sciences

🇮🇳

New Delhi, Delhi, India

© Copyright 2025. All Rights Reserved by MedPath