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Short Term Effect of Glucocorticoids on Brown Adipose Tissue Thermogenesis in Humans

Phase 4
Completed
Conditions
Brown Adipose Tissue
Interventions
Drug: Placebo
Drug: Prednisone
Registration Number
NCT03269747
Lead Sponsor
University Hospital, Basel, Switzerland
Brief Summary

Interventional, Placebo controlled cross-over study to investigate the short-term effects of glucocorticoids (prednisone) on human brown adipose tissue.

Detailed Description

Active brown adipose tissue (BAT) has recently been unambiguously discovered in human adults. Active BAT increases energy expenditure and improves glucose tolerance. Pharmacological use of glucocorticoids (GCs) is widespread in clinical practice due to their high anti-inflammatory efficacy. While short-term administration even of high doses usually is well tolerated, long-term use of medium to high amounts of GCs leads to unfavorable metabolic changes, characterized by an increase in intra-abdominal fat mass, a decrease in muscle mass and insulin resistance.

In line with these well-known side-effects of GCs, several in vitro studies and animal models demonstrate an inhibiting effect of GCs on BAT thermogenesis.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
16
Inclusion Criteria
  • Healthy male volunteers
  • BMI between 19-27 kg/m2
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Exclusion Criteria
  • Cold induced thermogenesis of less than 5% basal metabolic rate (determined during screening visit)
  • Contraindications to the class of drugs under study, e.g. known hypersensitivity or allergy to class of drugs or the investigational product,
  • History of depressive disorder, anxiety disorder
  • History of tuberculosis or latent infection
  • Increased intraocular pressure
  • History of peptic / gastrointestinal ulcer disease
  • Concomitant medication: Non-steroidal anti-inflammatory drugs (NSAID), other glucocorticoids, diuretics, antihypertensives, fibrates or statins, metformin
  • Other clinically significant concomitant disease states (e.g., renal failure, hepatic dysfunction, cardiovascular disease, diabetes mellitus),
  • Hypersensitivity to cold (e.g. Raynaud Syndrome)
  • Allergy to local anesthetic
  • Known or suspected non-compliance, drug or alcohol abuse,
  • Inability to follow the procedures of the study
  • Participation in another study with investigational drug within the 30 days preceding and during the present study,
  • Previous enrolment into the current study,
  • Enrolment of the investigator, his/her family members, employees and other dependent persons,
  • Hypothyroidism without sufficient substitution
  • Claustrophobia
  • MRI incompatible implants
  • Enrolment into another study using ionizing radiation within the previous 12 months.
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Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
PlaceboPlaceboPlacebo daily for 7 days
PrednisonePrednisonePrednisone 40 mg daily for 7 days
Primary Outcome Measures
NameTimeMethod
Cold induced thermogenesisat the end of each treatment period (day 7). Prednisone vs. Placebo

: Increase in energy expenditure above resting metabolic rate in response to a mild cold stimulus determined by indirect calorimetry

Secondary Outcome Measures
NameTimeMethod
volume of supraclavicular BATat the end of each treatment period (day 7). Prednisone vs. Placebo

determined by MRI

cold stimulated FGD uptake in brown adipose tissueat the end of each treatment period (day 7). Prednisone vs. Placebo

determined as SUVmean by FDG-PET/CT

fat fraction of supraclavicular BATat the end of each treatment period (day 7). Prednisone vs. Placebo

determined by MRI

SUVmax in the supraclavicular adipose tissue depotat the end of each treatment period (day 7). Prednisone vs. Placebo

determined by FDG-PET/CT

Trial Locations

Locations (1)

University Hospital Basel, Department of Endocrinology

🇨🇭

Basel, BS, Switzerland

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