Repair, Remodeling and Regeneration of the Bronchial Epithelium of COPD Patients
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Enrollment
- 30
- Locations
- 2
- Primary Endpoint
- Full recovery one day after injury (yes / no) proportion of cells showing the repair, remodeling or regeneration of the epithelium (ciliated cells, mucus cells, Clara cells) in culture in air-liquid interface in the three groups
Study Overview
Brief Summary
COPD is characterized by exagerated decline FEV1 related to obstructive non reversible airflow. This could be the consequence of structural changes and inflammatory pattern of the bronchial wall. Lesions could lead to normal but also abnormal remodeling specially in COPD including a decrease in Club cells number and function.There is no treatment actually available targeted to a normal repair of the epithelium. The objective of this work is to identify potential targets for reprograming bronchial epithelial cells I order to achieve a good repair.
Study Design
- Study Type
- Interventional
- Allocation
- Na
- Intervention Model
- Single Group
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to — (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •COPD - Smokers or ex smokers > 30p/yr
- •FEV1/FVC < 0.7
- •With a fibroscopy prescription
- •Smokers - Smokers or ex smokers > 30p/yr
- •FEV1/FVC > 0.7
- •With a fibroscopy prescription
- •Controls - age superior or equal to 18
- •no smoker (for 5 years)
- •with a fibroscopy prescription
Exclusion Criteria
- •Xylocaine hypersensibility
- •Porphyria
- •severe hepatic failure
- •Severe cardiac failure
Arms & Interventions
Heathy volunteers, smokers and COPD
Intervention: In vitro experiments on bronchial epithelial cells (Other)
Outcomes
Primary Outcomes
Full recovery one day after injury (yes / no) proportion of cells showing the repair, remodeling or regeneration of the epithelium (ciliated cells, mucus cells, Clara cells) in culture in air-liquid interface in the three groups
Time Frame: 1 day
The study will be judged on the proportion of cells showing the repair, remodeling or regeneration of the epithelium (ciliated cells, mucus cells, Clara cells) in culture in air-liquid interface in the three groups.
Secondary Outcomes
- Measurement of apoptosis and inflammation (IL8)(24 months)
- Measure the effect of the CC10 protein secreted by Clara cells on the repair of the bronchial epithelium.(24 months)