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Platelet PAR-1 Activation in Health and Diabetes

Completed
Conditions
Type 2 Diabetes
Healthy
Registration Number
NCT04280549
Lead Sponsor
Vanderbilt University Medical Center
Brief Summary

Platelet activation has been associated with bad events like heart attack and stroke. There are a variety of platelet activators that regulate how active a platelet is. We are interested in Protease-activated receptors (PAR)-1. We are currently studying PAR-1 activation in persons with severe peripheral artery disease. We seek, through this project, to understand PAR-1 activation in persons without peripheral artery disease. As many patients with peripheral artery disease have diabetes, we will also evaluate PAR-1 activation in persons with type 2 diabetes. In addition we will assess the impact of the glucagon-like peptide (GLP)-1 signaling pathway on platelet activation. Levels of platelet activation will be determined using platelet aggregation experiments and assessment of platelet-monocyte aggregates in peripheral blood.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
100
Inclusion Criteria
  • Healthy men and women ≥60 years of age OR
  • Type 2 diabetic men and women ≥60 years of age
Exclusion Criteria
  • Active cancer
  • Prior myocardial infarction, prior stroke, diagnosed peripheral artery disease
  • Severe liver (cirrhosis, cancer, or end-stage liver disease), kidney disease (eGFR <30 cc/min).
  • Pregnancy or lactation
  • Active vasculitis
  • Anticipated lifespan < 2 years
  • Current use of a DOAC

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Platelet ActivationOne day

Number of dilysyl-MDA cross-links formed in human platelets

Protease Activated Receptor-1 ActivationOne day

Number of cleaved PAR-1 receptors in proportion to number of uncleared PAR-1 receptors

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Vanderbilt University Medical Center

🇺🇸

Nashville, Tennessee, United States

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