Studies on the Complex Molecular Etiology and Cellular Landscape of Hip Osteoarthritis
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 110
- 试验地点
- 4
- 主要终点
- Characterization of cell populations in OA
研究概览
简要总结
The purpose of this study is to cast light on the highly complex etiology and cellular landscape of hip osteoarthritis by utilising single-cell and spatial omics.
详细描述
The specific objectives of this project are:
- Using the latest single-cell RNA sequencing (scRNAseq) techniques the investigators aim to A) characterize what kind of cell populations are found in different synovial tissues and blood derived samples of OA patients, B) determine how the cell composition differs between arthritic and corresponding non-arthritic tissues, C) map the transcriptional and regulatory landscape of the cells mentioned in A and B focusing on the inflammatory responses, D) determine what are the key molecular pathways activated in OA.
- To determine if some of the blood-derived immune cell populations or their products could be used as biomarkers for OA.
- To map the whole transcriptome and proteome of OA and non-arthritic control tissue while keeping the morphological context with spatial omics technologies.
- Further differentiation and identification of OA endotypes utilizing the single-cell and spatial omics data.
The project includes a Rheumatoid sub-study where the main objective is to compare arthritic tissue and peripheral blood constituents between OA and rheumatoid arthritis patients to explore the differences in the disease mechanisms.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 74 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- 未提供
排除标准
- •The body mass index must be below 35
- •Age < 18 or > 74
- •The OA patients may not have diabetes, rheumatoid arthritis (RA), or metabolic syndrome.
- •For the Rheumatoid sub-study, the exclusion criteria are the same as above except for the RA.
结局指标
主要结局
Characterization of cell populations in OA
时间窗: Starting during the first quarter of 2025, ending by the last quarter of 2026.
Characterization of cell populations found in different synovial tissues and blood derived samples of OA patients utilising single-cell RNA sequencing solutions.
Comparison of cell populations between OA cases and controls
时间窗: Starting during the first quarter of 2025, ending by the last quarter of 2026.
The investigators will determine how the cell composition differs between arthritic and corresponding non-arthritic tissues utilising single-cell RNA sequencing solutions.
Cellular landscape in OA
时间窗: Starting during the last quarter of 2024, ending by the last quarter of 2026.
The investigators will map the transcriptional, regulatory and protein landscape of OA at single-cell and tissue (spatial) level.
Key molecular pathways of OA
时间窗: Starting during the last quarter of 2025, ending by the last quarter of 2027.
The investigators will determine what are the key molecular pathways activated in OA.
Comparison of disease mechanisms between RA and OA
时间窗: Starting during the last quarter of 2024, ending by the last quarter of 2028.
In the Rheumatoid sub-study the investigators will explore the differences in the disease mechanisms between OA and RA by comparing synovial tissues and peripheral blood sample constituents.
次要结局
- Biomarkers for OA(Starting during the second half of 2026, ending by the last quarter of 2028.)
- OA endotypes(Starting during the first half of 2025, ending by the second half of 2027.)
研究者
Lea Mikkola
Principal Investigator
University of Turku