Testing SIROLIMUS in Beta-thalassemia Transfusion Dependent Patients
- Registration Number
- NCT03877809
- Lead Sponsor
- Rare Partners srl Impresa Sociale
- Brief Summary
Beta-thalassemias are hereditary blood disorders caused by reduced or absent synthesis of hemoglobin beta chains, with variable outcomes ranging from severe anemia to clinically asymptomatic individuals. Treatment is symptomatic and thalassemia is a major unmet medical need. Survival is increased, even in patients needing transfusions, in comparison with a few years ago, but the quality of life is poor for many patients. In some patients, an anomalous expression of gamma-globin genes has been observed, with a consequent rise in Fetal Hemoglobin levels. The patients displaying a clinical phenotype known as Hereditary Persistence of Fetal Hemoglobin (HPFH) exhibit a positive clinical status. To mimick HPFH, several compounds able to induce expression of fetal hemoglobins (HbF) have been evaluated. Within this framework, sirolimus is particularly interesting as an inducer of HbF. It has been used for many years for different indications and the available preclinical evidence warrant the start of a clinical development plan in thalassemia. The investigators propose a clinical trial in beta-thalassemia patients, designed to evaluate the effect of sirolimus on several parameters related to red blood cell status and to the level of HbF in particular, as a first step for the full clinical development in this new indication.
- Detailed Description
The general aim of the protocol is to demonstrate the applicability of a personalised and precision medicine approach in beta-thalassemia in a clinical trial setting for a repurposed drug, namely sirolimus. The presence of high level of Fetal Hemoglobin (HbF) is considered a condition predictive of a favourable outcome in thalassemia and its increase induced by pharmacological agents is considered a potential way to improve clinical status of the patients. In the present trial, in terms of efficacy analysis, the investigators will focus their attention on HbF levels.
Primary objective:
• To evaluate the suitability of sirolimus for the treatment of beta-thalassemia patients within the frame of a comprehensive project aimed to the reduction of their transfusions need (consequently ameliorating their quality of life). This goal can be obtained through a pharmacologically mediated increased level of HbF, with a prerequisite to be verified, namely the correlation between induction of HbF in vitro and in vivo in single patients.
Secondary objectives:
* To assess safety of sirolimus and correlation between administered dose and blood levels in beta-thalassemia patients,
* To assess the influence of sirolimus on transfusion regimen
* To assess the effect of sirolimus on hematopoietic and immune system of thalassemia patients.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 26
Not provided
- Patient treated with hydroxyurea at selection visit or in the last 6 months;
- Ongoing treatment with drugs possibly affecting sirolimus actions;
- Documented aspartate aminotransferase (AST) or alanine aminotransferase (ALT) > 3x Upper Limit of Normal (ULN) at selection;
- Documented Platelet count <150.000/microliter and >1.000.000/microliter at selection;
- Heart failure as classified by the New York Heart Association (NYHA) classification 3 or higher;
- Uncontrolled hypertension defined as systolic blood pressure (BP) ≥ 140 mm Hg or diastolic BP ≥ 90 mm Hg;
- Significant arrhythmia requiring treatment,
- Corrected QT interval> 450 msec on selection ECG;
- Ejection fraction <50% by echocardiogram, multiple gated acquisition scan or cardiac magnetic resonance;
- Myocardial infarction within 6 months prior of selection;
- Positivity for human immunodeficiency virus (HIV) antibody, active hepatitis B (HBV) or hepatitis C (HCV) as demonstrated by the presence of hepatitis B surface antigen (HBsAg) and a positive HCV-RNA test, HBcAb and HBV-DNA positivity
- White blood cell [WBC] count <3000 cells per μL and/or Granulocytes <1500/mm3;
- Total cholesterol > 240 mg/dl;
- Triglycerides > 200 mg/dl;
- Proteinuria with urinary protein >1g/24 hrs;
- Current participation in another trial with investigational drug or experimental device, or inclusion in another trial with investigational drug or experimental device within the preceding month;
- Major surgery (including splenectomy) within 60 days before selection (patients must have fully recovered from any previous surgery);
- Iron chelation therapy changed in the last 3 months prior to selection (note that Deferiprone is not accepted as a chelation therapy drug in this study while Desferioxamine and Deferasirox are tolerated at stable dose);
- Current treatment with macrolide antibiotics (clarithromycin);
- Pregnant or lactating women;
- History of severe allergic or anaphylactic reactions or hypersensitivity to excipients in the experimental drug;
- Treatment with live vaccines within 90 days preceding the selection;
- Subject with history or current malignancies (solid tumors and haematological malignancies) or presence of masses/tumor detected by ultrasound at selection;
- Subject with any significant medical condition and/or laboratory abnormality considered by the investigator as not adequately controlled at the time of selection.
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Arm && Interventions
Group Intervention Description Open label trial Sirolimus 0.5 mg Sirolimus 0.5 mg tablets
- Primary Outcome Measures
Name Time Method Change from baseline of fetal hemoglobin level 360 days Fetal hemoglobin level in peripheral blood at day 360 compared to day 0, assessed through high pressure liquid chromatography (HPLC)
- Secondary Outcome Measures
Name Time Method Change from baseline of biomarkers for haemolysis 360 days - Evaluation of the biomarkers for haemolysis level at day 180 and 360 compared to baseline. Biomarkers will include: serum lactate dehydrogenase (LDH) level
Change from baseline of Iron status 360 days * Evaluation of the intake of iron chelators at days 180 and 360 compared to baseline
* Evaluation of serum ferritin level at day 90, 180 and 360 in comparison with day 0Change from baseline of biomarkers for erythropoiesis 360 days - - Evaluation of the biomarkers for erythropoiesis level at day 180 and 360 compared to baseline. Biomarkers will include: erythropoietin level, serum transferrin receptor level.
Change from baseline of γ-globin expression 360 days Level of induction of the γ-globin expression at day 90, 180 and 360 compared to day 0
Change from baseline of fetal hemoglobin level 180 days Fetal hemoglobin level in peripheral blood at days 90 and 180 compared to day 0, assessed through HPLC
Change from baseline of tranfusion needs 360 days - Measurement of the total blood quantity (in mL) transfused and recording of the number of transfusions done in a semester (day -360 to -180, day -180 to 0, day 0 to 180, day 180 to 360)
Change from baseline of Immune function 360 days * Peripheral blood immunophenotype-Lymphocyte subsets at day 90 and 360 compared to day 0
* Quantitative analysis of ImmunoglobulinG/ImmunoglobulinA/ImunoglobulinM at day 90 and 360 compared to day 0Change from baseline of Quality of Life 360 days Evaluation of the patient quality of life at 6 and 12 months compared to baseline through Transfusion-dependent Quality of Life questionnaire (TranQol), measuring specifically the quality of life in patients with thalassemia. The TranQol is a disease-specific Quality of Life measure that has been shown to be valid and reliable (Klaassen et al, British Journal of Haematology, 2014, 164, 431-437). On a total scale of 0-100, higher values always represent a better outcome. The questions are grouped into four domains: physical health, emotional health, family functioning, and school and career functioning. The adult self-report questionnaires include a fifth category on sexual activity which is only one item. Subscales are summed.
Trial Locations
- Locations (2)
Department of Growth and Reproduction Azienda Ospedaliero-Universitaria S.Anna
🇮🇹Ferrara, Italy
Department of Life Sciences and Biotechnology, Section of Biochemistry and Molecular Biology
🇮🇹Ferrara, Italy