MedPath

The Effect of n-3 Polyunsaturated Fatty Acid Supplements in Patients With Non-alcoholic Fatty Liver Disease

Phase 2
Completed
Conditions
Non-alcoholic Fatty Liver Disease
Interventions
Dietary Supplement: Efamax
Registration Number
NCT00819338
Lead Sponsor
University of Nottingham
Brief Summary

The principal purpose of this study is to determine whether increased intakes of n-3 polyunsaturated (omega-3) fatty acids will reduce the amount of fat stored in the liver in patients with non-alcoholic fatty liver disease.

Detailed Description

Non-alcoholic fatty liver disease (NAFLD) is present in 10-24% of the general adult population. The first step of NAFLD involves the accumulation of fat within the liver (steatosis). Steatosis occurs either due to defective generation, metabolism or excretion of fatty acids by the liver. The next step in NAFLD progression is inflammation, which commonly occurs due to pro-inflammatory stimuli. Persistent inflammation results in end-stage liver disease. NAFLD is associated with the metabolic syndrome, which is characterised by central obesity, insulin resistance, raised triglycerides and hypertension. With the current obesity epidemic, there is predicted to be greater numbers of patients with NAFLD in the future.

Polyunsaturated fatty acids (PUFAs) are essential components of our diet, though standard Western intakes are lower than the recommended amounts. Supplementing the long chain n-3 PUFAs (commonly termed omega-3), EPA and DHA, improves many of the metabolic syndrome features. They lower plasma triglycerides, and may improve insulin resistance.

The diet of NAFLD patients tends to be deficient in n-3 PUFAs and have an excessive intake of the harmful n-6 PUFAs. This pattern is mirrored in their liver lipid content as assessed at biopsy.

Currently there is no proven treatment for NAFLD. Animal studies and limited studies in patients have been supportive of a benefit with n-3 polyunsaturated fatty acids. This needs to be further assessed.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
58
Inclusion Criteria
  1. Age greater than 18 years
  2. Liver biopsy diagnosis of NAFLD
Exclusion Criteria
  1. Excessive alcohol intake - > 21 units per week in men and > 14 in women
  2. A further liver disease diagnosis
  3. Poorly controlled diabetes - HbA1c > 8.0%, or use of insulin sensitisers
  4. Pregnancy
  5. Cirrhosis
  6. Contraindications to MR scanning - pacemaker or metallic foreign body etc.
  7. Changes in the dose or initiation of lipid altering medication within the preceding three months, such as statins, fibrates or systemic steroids
  8. Use of n-3 PUFA supplements within the prior 4 months, an adequate washout period
  9. Significant co-morbid inflammatory illnesses as determined by research team

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
polyunsaturatedEfamax5g per day of polyunsaturated fatty acids (3.5g EPA and DHA).
monounsaturatedEfamax5g a day of oleic enriched sunflower oil
Primary Outcome Measures
NameTimeMethod
Reduction of intrahepatic fat content as determined by magnetic resonance spectroscopy3 months
Secondary Outcome Measures
NameTimeMethod
Serum liver function tests, lipids, free fatty acids3 months
Insulin resistance as assessed by HOMA-IR and Adipose Tissue Insulin Resistance Index3 months
Liver saturated, monounsaturated and polyunsaturated fatty acid indexes as assessed by MR spectroscopy3 months
Visceral obesity as quantified by MRI, and the adipose derived serum leptin and adiponectin3 months
Primary assessment of the fibrotic and inflammatory status of the liver with serum TGF beta, TNF a, IL-6, IL-8, IL-8, IL-103 months
Further informative cytokine analyses: GM-CSF, IFN-G, IL-1B, IL-1RA, IL-2, IL-4, IL-5, MCP13 months
Compliance assessed by serum phospholipid fatty acids3 months

Trial Locations

Locations (1)

Wolfson Digestive Diseases Centre, University Hospital

🇬🇧

Nottingham, United Kingdom

© Copyright 2025. All Rights Reserved by MedPath