Transporte Eritrocitario de Lactato en Ejercicio
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 84
- 试验地点
- 4
- 主要终点
- Level of Monocarboxylate Transporter 1 in erythrocytes membrane
研究概览
简要总结
The goals of this clinical trial are:
- to study lactate kinetic between plasma and erythrocytes during an intervallic exercise and its subsequent recovery, considering the blood pH, the genotype for the T1470A polymorphism of the SLC16A1 gene (rs1049434), and the amount of MCT1 in erythrocytes membrane;
- to analyze the levels of MCT1 in the erythrocytes membrane according to training status and genotype for the T1470A polymorphism of SLC16A1 (rs1049434).
For this, the project will have two phases:
- In phase I, trained participants will perform one maximal incremental test and one intervallic submaximal test with a final active recovery.
- Phase II, levels of the MCT1 protein in the erythrocyte membrane will be quantified from trained and sedentary participants.
详细描述
Introduction
Lactate is the end product of glycolysis and has the capacity to be transported from one cell to another, performing numerous functions. This transport is mainly through several monocarboxylate transporters (MCT). The most predominant is MCT1, which transfers lactate bidirectionally depending on the lactate concentration and pH on each side of the membrane. MCT1 is ubiquitous, being present in most human tissues like the erythrocyte membrane, indicating that these cells are capable of carrying lactate throughout the bloodstream. Some studies have described lactate kinetics between the different blood compartments (erythrocyte-plasma) during incremental and steady-state exercises, but not during intermittent exercise and/or recovery after exercise. Moreover, several studies have observed that the function of MCT1 is conditioned by the presence of a genetic variant: the T1470A polymorphism (rs1049434), which involves a substitution of aspartic acid for glutamic acid at position 490 of the protein. Data suggest that an AA (Glu/Glu) genotype for this single nucleotide polymorphism (or SNP) leads to a higher lactate transport capacity than the TT (Arg/Arg) profile. However, no study has analyzed if this SNP directly affects erythrocyte MCT1 function or content Therefore, the two general objectives of this clinical trial are:
- to study lactate kinetic between plasma and erythrocytes during an intervallic exercise and its subsequent recovery, considering the blood pH, the genotype for the T1470A polymorphism of the SLC16A1 gene (rs1049434), and the amount of MCT1 in erythrocytes membrane;
- to analyze the levels of MCT1 in the erythrocytes membrane according to training status and genotype for the T1470A polymorphism of SLC16A1 (rs1049434).
Procedures
The study will be divided into two phases, each of which will be primarily oriented toward achieving one of two general objectives:
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •BMI between 18 and 25 kg/m2
- •Not smoker
- •In the case of trained participants: training at least 5 hours/week, of which at least 3 must be aerobic/anaerobic endurance training. Presentation of a medical certificate attesting to their medical fitness (capability) to perform intense physical exercise.
- •In the case of sedentary participants, less than 1 day of training per week or less than 2 hours of structured exercise per week.
排除标准
- •Presenting any pathology that could impede the correct performance of the tests, or could influence the main study variables.
- •In the case of the female sample, having irregular menstrual cycles, oligomenorrhea or amenorrhea.
结局指标
主要结局
Level of Monocarboxylate Transporter 1 in erythrocytes membrane
时间窗: Through study completion, average of 5 years, an average of 2 months per participant
Protein abundance of the monocarboxylate transporter 1 in erythrocyte membranes. It is measured using flow cytometry, from venous blood extracted from the forearm in rest state.
Plasma-Erythrocyte lactate difference
时间窗: Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years
Difference between lactate concentration inside the erythrocytes and lactate concentration in plasma, expressed in mMol/L (Millimoles Per Liter), from venous blood extracted during the intervallic test from the antecubital vein.
次要结局
- Erythrocyte lactate concentration(Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years)
- Plasma lactate concentration(Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years)
- Blood pH(Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years)
- Blood lactate concentration(Through Phase I of the project, only in the intervallic submaximal test. Average of 3 years)
研究者
Rocío Cupeiro, PhD
Physical Activity and Sports PhD (Associate Professor)
Technical University of Madrid