Comparative Evaluation of Antimicrobial Efficacy, Clinical and Radiographic Success of Calcium Hydroxide, Chlorhexidine, and Modified Triple Antibiotic Paste in Different Combination Forms As Intracanal Medicaments Against Enterococcus Faecalis in Primary Teeth
- Conditions
- Apical PeriodontitisNecrotic Primary MolarsPeriapical Pathosis of Primary Molars
- Interventions
- Combination Product: calcium hydroxide mixed with saline as intracanal medicationCombination Product: triple antibiotic paste (TAP) mixed with chlorehexidineCombination Product: Triple Antibiotic Paste mixed with salineCombination Product: Calcium Hydroxide mixed with chlorohexidine Intracanal medication
- Registration Number
- NCT06625645
- Lead Sponsor
- Mahy Abdel Raouf Aly Kamar
- Brief Summary
Comparing between different intracanal medications with different combination forms of Modified Triple Antibiotic Paste, Calcium Hydroxide and Chlorehexidine, regarding antimicrobial efficacy through bacterial reduction count of enterococcus Faecalis and regarding clinical and radiographic success in non vital primary teeth necessitating pulpectomy with follow up time 1 year.
- Detailed Description
1. Full medical and dental history will be obtained from the parents of the patients participating in this study.
2. A thorough clinical and radiographic examination of the tooth to be treated will be done.
3. The tooth will be anaesthetized using the inferior alveolar nerve block technique for lower molars and infiltration for upper molars
4. Before isolation, antisepsis of the oral cavity will be performed by rinsing for 1 min with 10 mL chlorhexidine gluconate mouth-wash 0.2 %.
5. The tooth will be properly isolated using rubber dam.
6. Access cavity will be performed using a round bur.
7. The root canals will be irrigated with 1 ml sterile saline solution.
8. Patency of the root canal will be obtained using stainless steel hand k- files size #15
9. Working length will be determined using apex locator
10. The first microbial samples (S1) will be collected to assess bacterial count of the root canals using 1 sterile paper point size 20, which will be inserted into the widest root canal for 1 min with pumping movements (McGurkin-Smith et al., 2005)
11. It will be placed inside sterile tubes containing a reduced transport medium of thioglycolate.
12. The canals will be thoroughly irrigated using 3ml syringe of 1.25% Sodium hypochlorite. A side-vented needle will be used to control the possibility of irrigant apical extrusion.
13. Mechanical preparation will be performed with rotary system for all the cases.
14. Irrigation with saline solution will be used to neutralize all the previously used solutions.
15. Then, a second microbial samples (S2) representing the antibacterial effect of the mechanical preparation will be obtained with the same procedure as the first sample (S1).
16. According to the randomization sequence, patients will be assigned to one of 4 groups:
1- A mix of modified triple Antibiotic Paste (M-TAP) and normal saline (experimental).
2- A mix of Ca(OH)2 and 2% CHX gluconate solution(experimental). 3- A mix of M-TAP and 2% CHX gluconate solution(experimental). 4- A mix of Ca(OH)2 and normal saline (control) 17) The canals will be dried and filled with intracanal medication plugged into the canal by using a plugger.
18) At the end of the first visit a piece of sterile cotton will be placed in the pulp chamber.
19) All teeth will be dressed with intermediate restorative material (IRM) as a temporary filling, in order to ensure proper sealing with no leakage of any oral fluids inside the root canal, which might disturb the action of the intracanal medication.
20) After 7 days, the temporary restoration will be removed. 21) Disinfection of the operative field will take place as performed previously using the same protocol.
22) Irrigation of the root canals with 2 mL of saline to flush out the medicaments and the third bacteriological sample (S3) will be taken out.
23) Later, canals will be filled with Metapex (Metabiomed, Korea), followed by the placement of stainless-steel crowns (3M ESPE).
24) The samples will be placed in sterile vials with thioglycolate (transport medium). The vials will be sealed tightly to avoid contamination and labeled. The labeled vials will be kept in a freezer and will be sent to the laboratory within 1 hour of collection, and samples will be assessed for total E. faecalis count (Qamar et al., 2023).
Recruitment & Eligibility
- Status
- NOT_YET_RECRUITING
- Sex
- All
- Target Recruitment
- 32
• Primary molars necessitating pulpectomy diagnosed with deep dental caries with pulp necrosis, apical periodontitis and periapical pathosis or both.
- Non-Vital pulps.
- Negative response to hot or cold pulp tester (ethyl chloride spray ).
- Decayed tooth with spontaneous unprovoked long dull aching pain showing signs of necrosis of the pulp
- Medically compromised and uncooperative children
- Tooth indicated for extraction
- Mobile teeth (Miller's Grade 2 or more)
- teeth with more than two-thirds of root resorption
- previous endodontic treatment
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Calcium Hydroxide mixed with saline as control intracanal medication calcium hydroxide mixed with saline as intracanal medication using calcium hydroxide with saline as control intracanal medication for necrotic primary molars different types and combinations of intracanal medications for necrotic primary molars triple antibiotic paste (TAP) mixed with chlorehexidine - different types and combinations of intracanal medications for necrotic primary molars Triple Antibiotic Paste mixed with saline - different types and combinations of intracanal medications for necrotic primary molars Calcium Hydroxide mixed with chlorohexidine Intracanal medication -
- Primary Outcome Measures
Name Time Method Enterococcus faecalis bacterial reduction 3 times, one after the access and gaining patency, second one after chemomechanical preparation and the third one after 7 days from intracanal medication insertion in the canal Samples from canal using paper point size 20 with pumping action from the widest canal three times one after the access and gaining patency, second one after chemomechanical preparation and the third one after 7 days from intracanal medication insertion in the canal, then the samples will be placed in sterile vials with thioglycolate (transport medium). The vials will be sealed tightly to avoid contamination and labeled. The labeled vials will be kept in a freezer and will be sent to the laboratory within 1 hour of collection, and samples will be assessed for total E. faecalis count.
- Secondary Outcome Measures
Name Time Method Clinical success 3,6 and 12 months measuring pain through presence or absence of pain Visual inspection of any changes (Redness or swelling of vestibular area (presence or absence of any change) Tenderness upon percussion ( positive or negative response) Tenderness upon touching of the vestibulum ( positive or negative response)