Effect of Probiotics on Chromogenic Bacteria and Extrinsic Stains in Children
试验速览
- 阶段
- 4 期
- 状态
- 已完成
- 入组人数
- 9
- 试验地点
- 1
- 主要终点
- chromogenic bacterial count before and after probiotic application
研究概览
简要总结
To investigate the bactericidal effect of probiotics (Streptococcus salivarius M18 and Lactobacillus reuteri) on two types of bacteria (Aggregatibacter actinomycetemcomitans and Actinomyces naeslundii) causing black stains of teeth in vitro.
详细描述
In vitro:
An unstimulated salivary sample will be taken from patients having black stains. The two types of bacteria of each condition will be cultured, amplified, and measured in the laboratory from the saliva of these patients. After that, one type of probiotics will be applied to these bacteria separately and the same bacterial measurement will be done to evaluate the bactericidal effect of the probiotics on these bacteria, aiming to find the minimal inhibitory concentration of the probiotics.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 6 Years 至 14 Years（Child）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Patients with black stains on teeth scored via modified Lobene index scores 1-
- •Medically free patients
排除标准
- •Stains other than black.
- •Patients on antibiotic treatment or any other medication for the last 2 weeks.
- •Patients allergic to prophylaxis powder.
- •Parents refusing to participate
研究组 & 干预措施
before and after probiotic administration
all patients will submit an unstimulated salivary sample of 1ml will in sealed test tube
- then the patients will receive probiotics for 14 days .
- after 14 days and another salivary sample will be collected
干预措施: Probiotic with Prebiotic (Drug)
结局指标
主要结局
chromogenic bacterial count before and after probiotic application
时间窗: two weeks
bacterial count
次要结局
未报告次要终点