Effect of Total Sleep Deprivation on Vascular Function
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 23
- 试验地点
- 1
- 主要终点
- Endothelial function
研究概览
简要总结
Insufficient sleep is associated with an increased risk for cardiovascular disease. The causal mechanisms are currently unknown, but may include endothelial dysfunction. The purpose of this study is to examine the influence of sex and aging on the effects of total sleep deprivation on vascular function and whether exercise training attenuates these effects.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Males and females
- •20 to 30 and 50 to 60 years of age
- •Females will be eumenorrheic at enrollment or postmenopausal for at least 1 year
- •No major clinical disease (e.g., diabetes, cardiovascular, liver or chronic kidney disease) to minimize confounding of vascular measures
- •Participants will be sedentary (≤3x per week of ≤30 minutes of aerobic exercise/session) or trained (≥5x per week of moderate/vigorous aerobic exercise training)
排除标准
- •Age <20 or 31 to 49 or >60 years
- •Body mass index ≥30 kg/m^2 because obesity may affect vascular function
- •Use of medication that may affect vascular measures
- •Hormone replacement therapy or hormonal contraceptives within past year
- •Use of tobacco products (chewing tobacco, traditional or e-cigarettes) because they may influence vascular function
- •Being perimenopausal, pregnant or lactating because may influence vascular function
- •Being a shift worker because habitual sleep deprivation and altered circadian rhythm may influence vascular function.
- •Not having an "intermediate" chronotype based on the Morningness-Eveningness Questionnaire (MEQ; score <31 or >69) because this would influence the effect of overnight sleep deprivation on vascular function.
- •Sleep complaints based on the Pittsburgh Sleep Quality Index (PSQI; score >5) because this would influence the effect of overnight sleep deprivation on vascular function.
研究组 & 干预措施
Group A
Participants will have vascular function assessed following total sleep deprivation
干预措施: Supervised Total Sleep Deprivation (Other)
Group B
Participants will have vascular function assessed following a full night of sleep
Group C
Participants will have vascular function assessed following total sleep deprivation preceded by exercise
干预措施: Exercise (Other)
Group C
Participants will have vascular function assessed following total sleep deprivation preceded by exercise
干预措施: Supervised Total Sleep Deprivation (Other)
Group D
Participants will have vascular function assessed following a full night of sleep
结局指标
主要结局
Endothelial function
时间窗: through study completion, an average of 1 month
Endothelial function will be determined using brachial artery flow-mediated dilation via high resolution duplex ultrasonography. Reactive hyperemia will be produced by inflating a forearm cuff to \~250 mmHg for 5 minutes followed by rapid deflation.
Microvascular function
时间窗: through study completion, an average of 1 month
Microvascular function will be assessed using the forearm blood flow response to submaximal handgrip exercise. Brachial artery blood flow and diameter will be assessed using Doppler ultrasound.
次要结局
- Central arterial stiffness(through study completion, an average of 1 month)
- Central arterial hemodynamics(through study completion, an average of 1 month)