Modeling the Neurological Basis and Characterizing the Neurological Phenotype of Obesity Using Human Neural Stem Cells
试验速览
- 阶段
- 不适用
- 状态
- 终止
- 入组人数
- 6
- 试验地点
- 1
- 主要终点
- Generate human cell based models of obesity
研究概览
简要总结
This study aims to characterize the neurological basis of obesity and response to surgical and medical treatment by inducing adult pluripotent stem cells into neuronal cells from subjects that have demonstrated extreme response to bariatric surgery or pharmacological treatment for obesity.
详细描述
The investigators will consent subjects who have achieved extreme response to either bariatric surgery or pharmacologic treatment for obesity and harvest fibroblasts from skin biopsies, which will be reprogrammed to induced pluripotent stem cells (iPSC). These iPSC's will then be differentiated into neural progenitor cells, neurons, astrocytes, and microglia to identify genetic and epigenetic pathways altered in disease-specific neural progenitor cells of the brain.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 90 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •History of obesity
- •Treatment with bariatric surgery
- •Treatment with anti obesity medications
- •Greater than 70% excess weight loss at least 6 months after surgery
- •Greater than 15% weight loss on anti obesity medications
排除标准
- •Active cancer, not including non-melanoma skin cancer
- •Active eating disorder
- •Use of anti obesity medications in subjects with a history of bariatric surgery
- •Active complication of the upper GI tract in patients with a history of bariatric surgery
研究组 & 干预措施
Bariatric Surgery
Subjects that have demonstrated extreme response to bariatric surgery.
干预措施: Bariatric Surgery (Procedure)
Anti Obesity Medications
Subjects that have demonstrated extreme response to anti obesity pharmacotherapy.
干预措施: Anti Obesity Drugs (Drug)
结局指标
主要结局
Generate human cell based models of obesity
时间窗: 4 months
Fibroblasts will be expanded in culture and then reprogrammed to hiPSCs.
DNA sequencing
时间窗: 4 months
Perform DNA sequencing from skin biopsy progenitor cells
Identification of genetic and epigenetic pathways
时间窗: 4 months
Identify genetic and epigenetic pathways altered in disease-specific neural progenitor cells, neurons, and non-neuronal cells of the brain
Differentiation to human CNS cells
时间窗: 4 months
Disease specific hiPSCs cells will be differentiated into neural progenitor cells, neurons, astrocytes, and microglia
次要结局
未报告次要终点
研究者
Eduardo Grunvald
Clinical Professor of Medicine
University of California, San Diego