The Effect of Omega-3 Supplementation on Muscle Functioning, Inflammation and Muscle Signaling

Not Applicable

Completed

• Conditions: Aging
• Interventions: Other: Resistance exercise; Dietary Supplement: Omega-3 polyunsaturated fatty acid supplementation; Dietary Supplement: Placebo - appearance-matched oil supplementation

• Registration Number: NCT03986307
• Lead Sponsor: KU Leuven

Brief Summary

Elderly are supplemented with either Omega-3 (3 x 1.1g per day) or placebo (corn oil) during 14 weeks. The last 12 weeks, supplementation is combined with resistance exercise training (3x per week) focusing on upper leg strength. In the present project, the investigators study whether differences in muscle strength and/or muscle mass between conditions can be explained by omega-3-induced effects on systemic or muscle inflammatory signaling, or differences in muscle molecular signaling.

Detailed Description

Elderly are supplemented with either Omega-3 (3 x 1.1g per day) or placebo (corn oil) during 14 weeks. The last 12 weeks, supplementation is combined with resistance exercise training (3x per week) focusing on upper leg strength. In the present project, the investigators study whether differences in muscle strength and/or muscle mass between conditions can be explained by omega-3-induced effects on systemic or muscle inflammatory signaling, or differences in muscle molecular signaling.

Study Timeline

• Study Start: 2019-01-01
• Primary Completion: 2019-05-25
• Study Completion: 2019-05-25
• Status Verified: 2019-06
• Study First Submitted: 2019-06-04
• Study First Submitted QC: 2019-06-12
• Last Update Submitted: 2019-06-12
• Study First Posted: 2019-06-14
• Last Update Posted: 2019-06-14

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 23

Inclusion Criteria

• aged >= 65 years

Exclusion Criteria

• BMI < 20; BMI > 35
• regular NSAID intake in the last 3 months
• omega-3 supplementation in the last year
• regular resistance exercise in the last year
• cancer, liver and renal disease, unstable cardiovascular disease, musculoskeletal disease
• cognitive limitations that might interfere with the (adherence to) the intervention or with the testing of outcome parameters
• unstable body weight
• smoking

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Placebo	Resistance exercise	Elderly supplemented with corn oil.
Placebo	Placebo - appearance-matched oil supplementation	Elderly supplemented with corn oil.
Omega-3	Resistance exercise	Elderly supplemented with omega-3.
Omega-3	Omega-3 polyunsaturated fatty acid supplementation	Elderly supplemented with omega-3.

Primary Outcome Measures

Name	Time	Method
Isometric muscle strength	Change in muscle strength between prior to and following the study intervention of 14 weeks (Jan - May 2019)	Isometric muscle strength as assessed with Biodex Device in Nm
Systemic inflammatory signaling	Change in inflammatory signaling between prior to and following the study intervention of 14 weeks (Jan - May 2019)	Systemic (blood) markers of inflammation: hs-CRP (mg/L), IL-6 (pg/mL), TNFa (pg/mL)
Muscle strength	Change in muscle strength between prior to and following the study intervention of 14 weeks (Jan - May 2019)	One Repetition Maximum on the leg press fitness device in kg

Secondary Outcome Measures

Name	Time	Method
Muscle quality	Change in muscle quality between prior to and following the study intervention of 14 weeks (Jan - May 2019)	muscle strength (Torque) per muscle volume
Muscle molecular signaling (Protein levels of anabolic/catabolic signaling and skeletal muscle stress)	Change in muscle molecular signaling between prior to and following the study intervention of 14 weeks (Jan - May 2019)	Biochemical analyses of muscle biopsies obtained pre-post intervention in both conditions.; This involves parameters of muscle anabolism, catabolism, stress, structure, integrity, quality etc.: mTORC1 pathway, FoXO, MAPK (western blotting technique, values expressed as arbitrary units)

Trial Locations

Locations (1)

KU Leuven; 🇧🇪 Leuven, Belgium