Mechanistic and Molecular Study of the Process of Metastatic Dissemination in Colorectal Cancer
试验速览
- 阶段
- 不适用
- 入组人数
- 50
- 试验地点
- 2
- 主要终点
- Identification and characterization of the tumoral intermediaries on the peripheral blood
研究概览
简要总结
To confirm the role of the collective dissemination in the mechanisms of tumoral invasion of colorectal cancers
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Age > 18 years
- •Histological Diagnosis of stage IV colorectal cancer
- •Hepatic metastasis and/or peritoneal potentially resecable
- •Patient affiliated with a mode of the social security or recipient of such a mode
- •Information of the patient or his legal representative and collection of his assent
排除标准
- •None metastatic colorectal cancer
- •Cancer of appendicular origin
- •Patients deprived of liberty or unable to give his assent
研究组 & 干预措施
Patients with metastatic colorectal cancer
After inclusion in the study, eligible patients having hepatic metastases and/or péritonéales of a colorectal cancer considered as resecables will have biological and tissue samples.
The samples will be carried out at the time of the surgical gesture envisaged under general anaesthesia and will concern:
- Tumoral material: primitive tumour (if available), hepatic metastases and/or peritoneal
- Peritoneal liquid
- none tumoral peritoneum
- Portal blood
- Peripheral blood
- Cellulo-lymphatic material The necessary time to carry out the whole of these samples is estimated at 10-15 minutes maximum.
In the event of complex situations being able to complicate the surgical gesture initially envisaged or to increase by them morbidity, one or more these samples will not be carried out. This decision will be made at the discretion of the investigator.
干预措施: Sampling (Procedure)
结局指标
主要结局
Identification and characterization of the tumoral intermediaries on the peripheral blood
时间窗: Up to 24 months
After several separation and centrifugation steps, clusters and tumor cells are isolated and counted in the various biological samples. The different isolated intermediates will be analyzed by immunolabeling with the antibodies directed against CK20, EpCAM, Vimentine and CD45.
Identification and characterization of the tumoral intermediaries on the hepatic metastasis and/or peritoneal)
时间窗: Up to 24 months
After several separation and centrifugation steps, clusters and tumor cells are isolated and counted in the various biological samples. The different isolated intermediates will be analyzed by immunolabeling with the antibodies directed against CK20, EpCAM, Vimentine and CD45.
Identification and characterization of the tumoral intermediaries on the overflowing peritoneal
时间窗: Up to 24 months
After several separation and centrifugation steps, clusters and tumor cells are isolated and counted in the various biological samples. The different isolated intermediates will be analyzed by immunolabeling with the antibodies directed against CK20, EpCAM, Vimentine and CD45.
Identification and characterization of the tumoral intermediaries on the portal blood
时间窗: Up to 24 months
After several separation and centrifugation steps, clusters and tumor cells are isolated and counted in the various biological samples. The different isolated intermediates will be analyzed by immunolabeling with the antibodies directed against CK20, EpCAM, Vimentine and CD45.
次要结局
未报告次要终点