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Effects of Intermittent Caloric Restriction in Youth With Cardiometabolic Risk

Not Applicable
Completed
Conditions
Obesity
Overweight
Cardiometabolic Risk
Interventions
Behavioral: Low carbohydrate diet
Behavioral: Intermittent caloric restriction
Behavioral: Health education on reducing the cardiometabolic risk
Registration Number
NCT04011995
Lead Sponsor
Children's Hospital of Fudan University
Brief Summary

This is a randomized controlled pilot study to evaluate the effects of intermittent caloric restriction compared with low carbohydrate diet in youth with cardiometabolic risk.

Detailed Description

The National China Health and Nutrition Survey revealed that 42% of overweight children had at least one cardiovascular metabolic risk, such as hyperglycaemia, dyslipidemia or hypertension. Non-pharmaceutical life style modifications are the recommended intervention to these subjects, including caloric restriction and increased physical activity. However, the problems of poor compliance and metabolic adaptation are well known. Evidences from a number studies in rodent models and human indicated that intermittent caloric restriction (ICR) may stimulate series reactions in human body, including improvement of insulin sensitivity, blood pressure, oxidative stress and inflammation, and may be easier to follow. Evidence from larger human studies is strongly encouraged, while the effects, safety and adherence in chidren and youth need to be studied.

This study is a randomized controlled trial of intermittent caloric restriction versus low carbohydrate diet in youth with cardiometabolic risk over one month period. The intervention is based on the 5:2 diet, which involves caloric restriction for 2 days (consecutive or nonconsecutive, 600kcal/d for male and 500kcal/d for female) per week and unrestricted eating during the other 5 days of the week. For control group, the subjects receive 7 days (consecutive or nonconsecutive) of low carbohydrate diet intervention during the 14-day intervention period,. Carbohydrate intake of low-carbohydrate diet should be controlled as ≤ 50g per day. Total trial duration is one month consisting of a 14-day intervention phase and a 14-day self-maintenance phase. During the maintenance phase, two follow-up electronic questionnaires were conducted.

The study aim to explore effects of the dietary interventions on cardiometabolic markers, inflammatory reaction, oxidative stress and gut microbiome in youth with cardiometabolic risk.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
34
Inclusion Criteria

Have at least one of the following cardiometabolic abnormalities:

  1. Overweight or obesity (central obesity or general obesity)
  2. Prediabetes: impaired fasting glucose (IFG) and/or impaired glucose tolerance (IGT)
  3. Dyslipidemia
  4. Elevated blood pressure

Criteria for cardiometabolic abnormalities:

  1. Overweight or obesity

    For subjects (9 ≤ age ≤ 18 years) :

    • General obesity: Body mass index higher than the 85th percentile for overweight and the 90th percentile for obesity, based on the references for screening overweight and obesity in Chinese children and adolescents.

    • Central obesity: Waist circumference higher than the 90th percentile of the age and gender-specific reference for screening cardiovascular risk factors in Chinese children and adolescents.

    For subjects ( 19 ≤ age ≤ 30 years) :

    • General obesity: Body mass index between 24.0 and 27.9 kg/m2 for overweight and ≥28 kg/m2 for obesity.

    • Central obesity: Waist circumference ≥85cm for men and ≥80cm for female. Based on recommendation of overweight and obesity in Chinese adults.

  2. Prediabetes:

    With IFG and/or IGT. IFG: fasting glucose from 5.6 to 6.9 mmol/L; IGT: 2-h glucose in the 75g oral glucose tolerance test from 7.8 to 11.0 mmol/L.

    Based on recommendation of American Diabetes Association.

  3. Dyslipidemia:

    For subjects (9 ≤ age ≤ 18 years) :

    Triglycerides of ≥1.70 mmol/L or total cholesterol of ≥5.18 mmol/L or lowdensity lipoprotein cholesterol ≥3.37 mmol/L or highdensity lipoprotein cholesterol of ≤1.04 mmol/L. Based on recommendation in Chinese children and adolescents.

    For subjects ( 19 ≤ age ≤ 30 years) :

    Triglycerides of ≥1.7 mmol/L or total cholesterol of ≥5.2 mmol/L or lowdensity lipoprotein cholesterol ≥3.4 mmol/L or highdensity lipoprotein cholesterol of ≤1.0 mmol/L. Based on guideline for the management of dyslipidemia in Chinese adults.

  4. Elevated blood pressure:

For subjects (9 ≤ age ≤ 18 years) :

Blood pressure higher than the 90th percentile of blood pressure age and gender-specific reference standards for Chinese children and adolescents, or blood pressure >120/80 mmHg.

For subjects ( 19 ≤ age ≤ 30 years) :

Systolic blood pressure≥120 mmHg and/or diastolic blood pressure ≥80 mmHg. Based on Chinese guidelines for the management of hypertension.

Exclusion Criteria
  1. Diagnosis of severe diseases such as cardiac insufficiency, severe malnutrition or immunodeficiency.
  2. History of bariatric surgery.
  3. Use of antiobesity drugs or supplements.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Intermittent caloric restrictionHealth education on reducing the cardiometabolic risk-
Low carbohydrate dietLow carbohydrate diet-
Intermittent caloric restrictionIntermittent caloric restriction-
Low carbohydrate dietHealth education on reducing the cardiometabolic risk-
Primary Outcome Measures
NameTimeMethod
Reversal of cardiometabolic abnormalitiesFrom the baseline (the first day of the intervention phase) to the 14th day of the intervention phase.

The primary outcome is a composite outcome that will be measured by combining multiple reverse outcomes of the following cardiometabolic abnormalities: (1) overweight or obesity, (2) prediabetes, (3) hyperlipidemia and (4) elevated blood pressure. It is defined as occurring if any cardiometabolic abnormality has a reversal outcome at the 14th day of dietary intervention phase.

Reversal of cardiometabolic abnormalities is defined as:

At the 14th day of dietary intervention phase, at least one of the following indicators changed from abnormal at baseline to normal: overweight, obesity, prediabetes, hyperlipidemia and elevated blood pressure.

(The reversal of overweight or obesity is defined according to reference\[1, 2\]: For subject with general obesity at baseline: weight loss of 1.5 kg after intervention; For subject with central obesity at baseline: waist circumference reduction of 1.5 cm after intervention.)

Secondary Outcome Measures
NameTimeMethod
Change in insulinFrom the baseline (the first day of the intervention phase) to the 14th day of the intervention phase.

Serum from fasting blood sample is used for measurement of insulin.

Dietary energy intakeFrom the baseline (the first day of the intervention phase) to the 14th day of the intervention phase.

Daily dietary energy intake (kcals/day) is calculated by researchers using food weighing photos of the subject.

Change in monocyte chemoattractant protein-1 (MCP-1)From the baseline (the first day of the intervention phase) to the 14th day of the intervention phase.

MCP-1 will be measured by enzyme-linked immunosorbent assays (R\&D Systems, Inc. USA), using fasting serum samples at baseline and 14 days after intervention.

Change in insulin-like growth factor-1From the baseline (the first day of the intervention phase) to the 14th day of the intervention phase.

Fasting blood sample for measurement of insulin-like growth factor-1.

Change in gut microbial compositionsFrom the baseline (the first day of the intervention phase) to the 14th day of the intervention phase.

Stool sample of subject is used for measurement of gut microbial compositions.

Change in body weightFrom the baseline (the first day of the intervention phase) to the 14th day of the self-maintenance phase.

Body weight is measured by subject and reported by electronic questionnaire. It is measured to the nearest 0.1 kg.

Change in waist circumferenceFrom the baseline (the first day of the intervention phase) to the 14th day of the self-maintenance phase.

Waist circumference is measured by subject and reported by electronic questionnaire. It is measured to the nearest 0.1 cm using a constant tension tape around the waist at 1 cm below the navel as the subject exhaled. It is calculated as the average value of two measurements.

Self-reported dietary adherenceAt the 14th day of the self-maintenance phase.

Adherence to diet will be assessed with a electronic questionnaire in the self-maintenance phase.

Change in fibroblast growth factor 21 (FGF-21)From the baseline (the first day of the intervention phase) to the 14th day of the intervention phase.

FGF-21 will be measured by enzyme-linked immunosorbent assays (R\&D Systems, Inc. USA), using fasting serum samples at baseline and 14 days after intervention.

Change in free fatty acid (FFA)From the baseline (the first day of the intervention phase) to the 14th day of the intervention phase.

FFA will be measured by enzyme-linked immunosorbent assays (R\&D Systems, Inc. USA), using fasting serum samples at baseline and 14 days after intervention.

Change in mean blood glucoseFrom the baseline (the first day of the intervention phase) to the 14th day of the intervention phase.

Measured by a blood glucose monitor for 24-hour glucose monitoring during the 14-day intervention phase.

Trial Locations

Locations (1)

Children's Hospital of Fudan University

🇨🇳

Shanghai, China

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