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Analysing the Effect of Empagliflozin on Reduction of Tissue Sodium Content in Patients With Chronic Heart Failure

Phase 2
Completed
Conditions
Chronic Heart Failure
Interventions
Drug: Placebo Oral Tablet
Registration Number
NCT03128528
Lead Sponsor
University of Erlangen-Nürnberg Medical School
Brief Summary

The hypothesis is that the SGLT-2 inhibitor empagliflozin reduces tissue sodium content in patients with chronic heart failure, and if the hypothesis is proven, that this mechanism contributes to the beneficial effects found in EMPA-REG Outcome trial potentially via exerting beneficial effects on the vascular structure and function of the micro- and macrocirculation.

Detailed Description

SGLT-2 inhibitors such as empagliflozin inhibit the SGLT-2 transport in the proximal tubular cells of the kidney, thereby causing glucosuria to approximately 100 g per day (and sometimes even more). The SGLT-2 inhibition does not only cause glucosuria but also natriuresis, since with each molecule of glucose one molecule of sodium is inhibited to be reabsorbed. Indeed, during the first week SGLT-2 inhibition causes clinically detectable natriuresis but its effect in the long run is not yet illustrated. Of course, a new sodium balance will be achieved after a certain time (otherwise the human body would be completely salt depleted), but total sodium content could be different. With new innovative magnetic resonance imaging (MRI) technology we are able to assess tissue sodium content in the skin and muscle, and observed that sodium content is significantly increased with aging, severe hypertension or hyperaldosteronism. Furthermore, skin sodium content assessed by MRI was closely related to left ventricular mass (r=0.559, p\<0.0001, N=89) independently of age, gender, body mass index, and 24 h ambulatory blood pressure (β=0.343, p=0.001, N=89) 11. Using this technology, our first yet unpublished data (clinicaltrials.gov: NCT02383238) indicate that SGLT-2 inhibition decreases sodium content in the skin in patients with diabetes. Finally, we observed previously that in patients with acute chronic heart failure skin sodium content decreased from 43.5 mmol/l to 32.2 mmol/l after diuretic therapy.

Thus, the present study aims at analyzing changes in total and tissue sodium content after SGLT-2 inhibition with empagliflozin. In parallel, sodium intake and excretion and central systolic and pulse pressure as well as other vascular parameters will be assessed. In face of the upcoming studies with empagliflozin conducted in patients with reduced and preserved ejection fraction (two large-scale, prospective, doubleblind, placebo controlled studies planned by Boehringer Ingelheim as the sponsor), we thought that we focus on patients with chronic heart failure irrespective diabetic status. The hypothesis is that the SGLT-2 inhibitor empagliflozin reduces tissue sodium content in patients with chronic heart failure, and if the hypothesis is proven, that this mechanism contributes to the beneficial effects found in EMPA-REG Outcome trial potentially via exerting beneficial effects on the vascular structure and function of the micro- and macrocirculation.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
84
Inclusion Criteria
  • Age of 18 - 85 years
  • Male and Female patients (females of child bearing potential must be using adequate contraceptive precautions)
  • CHF (symptoms and/or sign of CHF, ejection fraction < 40% (HfrEF) 14 or symptoms and/or signs of CHF, ejection fraction 40-49 % and NT-pro BNP > 125 pg/ml, and at least one structural abnormality of left atrium or ventricle (HFmEF) 14 in stable conditions.
  • Females of childbearing potential or within two years of the menopause must have a negative urine pregnancy test at screening visit.
  • Informed consent has to be given in written form.
Exclusion Criteria
  • Any other form of diabetes mellitus than type 2 diabetes mellitus
  • Use of insulin or any SGLT-2 inhibitor within the past 10 weeks prior to the screening visit (visit 1).
  • Patients with more than two blood glucose lowering medications
  • Uncontrolled diabetes (fasting plasma glucose ≥ 240 mg/dl, HbA1c ≥ 10%)
  • Any history of stroke, transient ischemic attack, instable angina pectoris, or myocardial infarction within the last 6 months prior to study inclusion
  • Estimated glomerular filtration rate (eGFR) < 30 ml/min/1.73m² (following the inclusion criteria of EMPA-REG OUTCOME study 1-3)
  • Chronic heart failure NYHA stage IV
  • Use of loop diuretics above furosemide > 80 mg/day, or torasemide >40 mg/day, or piretanide > 6 mg/day
  • Implanted pacemakers or defibrillators
  • Any other relevant clinical contraindication of MRI examination
  • Uncontrolled arterial hypertension (i.e. ≥ 180/110 mmHg)
  • Severe disorders of the gastrointestinal tract or other diseases which interfere with the pharmacodynamics and pharmacokinetics of study drugs
  • Significant laboratory abnormalities such as Serum Glutamate-Oxaloacetate-Transaminase (SGOT) or Serum Glutamate-Pyruvate-Transaminase (SGPT) levels more than 3 x above the upper limit of normal range

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
EmpagliflozinEmpagliflozin 10mgPatients will be randomized to empagliflozin 10 mg orally once daily or one placebo tablet orally once daily.
Placebo Oral TabletPlacebo Oral TabletPatients will be randomized to empagliflozin 10 mg orally once daily or one placebo tablet orally once daily.
Primary Outcome Measures
NameTimeMethod
Skin sodium content14 weeks

Skin sodium content (23Na-MRI) assessed at the lower leg

Secondary Outcome Measures
NameTimeMethod
Muscle sodium content14 weeks

Sodium content of muscles

24-hour urine sodium excretion14 weeks

24-hour urine sodium excretion

Water content of skin and muscle14 weeks

Water content (1H) of skin and muscle

Sodium excretion14 weeks

Sodium excretion as assessed by sodium creatinine ratio in spot urine

N-terminal prohormone of brain natriuretic peptide14 weeks

N-terminal prohormone of brain natriuretic peptide (NT-pro-BNP) to assess their relation to change in tissue sodium content

HbA1c14 weeks

Diabetic control (e.g. fasting glucose, glycosylated hemoglobin \[HbA1c\])

Vascular stiffness Parameter (central systolic pressure)14 weeks

Vascular stiffness Parameter under resting conditions and ambulatory conditions and their association to change in tissue sodium content

Flow mediated vasodilation14 weeks

Flow mediated vasodilation (FMD) as measured by semiautomated ultrasound system

ABPM14 weeks

24-hour ambulatory blood pressure (ABP)

Visual analogue scale for dyspnea14 weeks

Visual analogue scale for dyspnea to assess their relation to change in tissue sodium Content.

Body constitution14 weeks

Body constitution (fluid status based on three compartment model lean body mass, adipose tissue mass and overhydration)

Vascular stiffness Parameter (Pulse pressure)14 weeks

Vascular stiffness Parameter under resting conditions and ambulatory conditions and their association to change in tissue sodium content

Body weight14 weeks

Measurement of body weight in kg

Trial Locations

Locations (1)

Clinical Research Center, Department of Nephrology and Hypertension, University of Erlangen-Nuremberg

🇩🇪

Erlangen, Germany

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