Effect of Supplementation With Anthocyanins Contained in Black Chokeberry (Aronia Melanocarpa) on Selected Indicators of Iron Metabolism in Female Swimmers
试验速览
- 阶段
- 1 期
- 状态
- 已完成
- 发起方
- 入组人数
- 22
- 试验地点
- 1
- 主要终点
- Physiological parameters of iron metabolism indicator V
研究概览
简要总结
The study included 8-weeks supplementation of black chokeberry extract with 18% standardization of the anthocyanins content (dose 2×200 mg per day), or a placebo product that was made from chokeberry fiber.
Purpose:
The aim of the study is effectiveness check of black chokeberry intake as a potential factor which, due to its ability to reduce inflammation and chelate iron ions, will have an impact on minimizing undesirable changes in the red blood cell system.
Hypothesis:
Main hypothesis: Supplementation of black chokeberry (Aronia melanocarpa) has a positive effect on iron economy of female swimmers.
Auxiliary hypotheses:
- Supplementation of black chokeberry enhances the antioxidant status and has a positive effect on the prooxidant-antioxidant balance.
- Supplementation of black chokeberry reduce inflammation induced by intensive exercises, by reducing inflammatory markers.
Study plan:
Before first term of the study:
Control of Menstrual Cycle Phases since March 2023 (installing the application on the phone - Menstrual Period Tracker calendar).
First term of the study:
- Menstrual cycle control survey.
- Conduct a body composition analysis.
- Collection of blood samples before the exercise test for biochemical determinations.
- Exercise test in the swimming pool - crawl style intensive test (800 m + 200 m + 50 m).
- Collection of blood immediately after exercise and 3 hours later.
- Supplementation for nearly 56 days, depending on the length of the menstrual cycle.
Second term of the study:
Repeat the measurements from the 1st date of test.
Participants: The group of selective and homogeneous female swimmers, who train swimming intensively and attend the Sports Championship School Complex.
详细描述
This study included 8-weeks supplementation of black chokeberry extract with 18% standardization of the anthocyanins content (dose 2×200 mg per day), or a placebo product that was made from chokeberry fiber. The first date of research completed the group of twenty-four and homogeneous female swimmers, who train swimming intensively and attend the Sports Championship School Complex. The second date of research completed the group of twenty-two selective and homogeneous female swimmers, who train swimming intensively and attend the Sports Championship School Complex.
Purpose:
The aim of the study is effectiveness check of black chokeberry intake as a potential factor which, due to its ability to reduce inflammation and chelate iron ions, will have an impact on minimizing undesirable changes in the red blood cell system.
Hypothesis:
Main hypothesis: Supplementation of black chokeberry (Aronia melanocarpa) has a positive effect on iron economy of female swimmers.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Outcomes Assessor)
入排标准
- 年龄范围
- 12 Years 至 19 Years（Child, Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Current medical examinations issued by a sports medicine doctor
- •Training experience a minimum of three years
- •Student of a sports championship school team
- •Consent of the athlete/legal guardian to participate in the research
- •Completion of the exercise test in the swimming pool - crawl style intensive test (800 m + 200 m + 50 m) on two test deadline
排除标准
- •Taking: oral contraceptive pills, supplements containing anthocyanin compounds during the study or a one month earlier
- •Health problems
- •Antibiotic therapy
研究组 & 干预措施
1. Supplemented group
The supplemented group (n=12) consumed capsules with highly concentrated 18% chokeberry extract (one capsule contained 200 mg). Each capsule also contained: chokeberry fiber, E460b magnesium salts of stearic acid (anti-caking agent), E551 silicon dioxide (anti-caking agent), hydrocypropyl methylcellulose (capsule shell) and E171 titanium dioxide (shell colour). The producer of all capsules is MLD Biotrade in Poznan.
The swimmers took 2 capsules a day (before breakfast and dinner) for nearly 56 days (depending on length menstrual cycle phase). The swimmers sipped each capsule with glass of water.
干预措施: Black chokeberry extract in capsules with 18% standardization of the anthocyanins content (Drug)
2. Placebo group
The control group (n=10) consumed capsules that were made from chokeberry fiber. The producer of all capsules is MLD Biotrade in Poznan.
The swimmers took 2 capsules a day (before breakfast and dinner) for nearly 56 days (depending on length menstrual cycle phase). The swimmers sipped each capsule with glass of water.
干预措施: Chokeberry fiber in capsules (Drug)
结局指标
主要结局
Physiological parameters of iron metabolism indicator V
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
ferritin \[ng/ml\]
Physiological parameters of blood morphology III
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
thrombocytes: PLT \[10\^3/mL\], MPV \[mm\^3\], PCT \[%\], PDW \[%\]
Physiological parameters of iron metabolism indicator III
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
transferrin \[mg/ml\]
Physiological parameters of blood morphology I
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
erythrocytes: RBC \[10\^6/mL\], HGB \[g/dL\], HCT \[%\], MCV \[mm\^3\], MCH \[pg\], MCHC \[g/dL\], RDW \[%\],
Physiological parameters of iron metabolism indicator I
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
iron \[µg/dl\]
Physiological parameters of iron metabolism indicator VI
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
erythroferrone \[ng/mL\]
Physiological parameters of iron metabolism indicator II
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
hepcidin \[ng/ml\]
Physiological parameter of hormonal indicator III
时间窗: At each exercise test (up to 15 minute before exercise)
luteinizing hormone (LH) \[mIU/ml\]
Physiological parameters of iron metabolism indicator IV
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
total iron-binding capacity (TIBC) \[µg/dl\]
Physiological parameters of immunological indicator II
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
interleukin 2 \[ng/L\]
Physiological parameters of immunological indicator IV
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
C-reactive protein \[mg/dL\]
Physiological parameter of hormonal indicator II
时间窗: At each exercise test (up to 15 minute before exercise)
estradiol \[pg/ml\]
Physiological parameters of blood morphology II
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
leukocytes: WBC \[10\^3/mL\], LYM \[10\^3/mL\], MON \[10\^3/mL\], GRA \[10\^3/mL\], LYM \[%\], MON \[%\], GRA \[%\],
Physiological parameters of antioxidant-prooxidant indicator
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
total antioxidant capacity \[mmol/l\]
Physiological parameter of hormonal indicator I
时间窗: At each exercise test (up to 15 minute before exercise)
progesterone \[ng/ml\]
Other physiological parameter I
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
myoglobin \[ng/ml\]
Physiological parameters of immunological indicator I
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
interleukin 6 \[ng/L\]
Physiological parameter of hormonal indicator IV
时间窗: At each exercise test (up to 15 minute before exercise)
follicle-stimulating hormone (FSH) \[mIU/ml\]
Other physiological parameter (with capillary blood - earlobe)
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
lactic acid \[mmol/l\]
Physiological parameters of immunological indicator III
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
interleukin 10 \[ng/L\]
Other physiological parameter II
时间窗: At each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after)
hypoxanthine \[mmol/l\]
次要结局
- Physiological parameters of iron metabolism indicator VII(Calculated with blood samples at each exercise test (up to 15 minute before exercise, up to 1 minute post exercise, 3 hours after))