Evaluation of Osteoanabolic effect of Doxycycline Proniosomal gel form : An invitro study.
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 发起方
- 入组人数
- 2
- 试验地点
- 1
- 主要终点
- Invitro evaluation of the biological behavior of doxycycline proniosomal gel for their use as bone regenerative material.
研究概览
简要总结
Evaluation of Osteoanabolic effect of Doxycycline Proniosomal gel form : An invitro study.
Objectives:
1.Invitro evaluation of the biological behavior of doxycycline proniosomal gel for their use as bone regenerative material.
2. To evaluate the cytotoxic effect of doxycycline proniosomal gel on pulpal dental stem cells by MTT assay.
3. To study the osteogenic differentiation potential of doxycycline proniosomal gel in mesenchymal stem cells by alizarin red staining.
4. To investigate the osteogenic gene expression such as alkaline phosphatase and osteonectin for osteogenic differentiation.
研究设计
- 研究类型
- Observational
入排标准
- 年龄范围
- 18.00 Year(s) 至 60.00 Year(s)（—）
- 性别
- All
入选标准
- •Healthy dental pulp mesenchymal stem cells procured from healthy non carious extracted tooth.
排除标准
- 未提供
结局指标
主要结局
Invitro evaluation of the biological behavior of doxycycline proniosomal gel for their use as bone regenerative material.
时间窗: 1,2and 6 months
次要结局
- 1. To evaluate the cytotoxic effect of doxycycline proniosomal gel on pulpal dental stem cells by MTT assay.(2. To study the osteogenic differentiation potential of doxycycline proniosomal gel in mesenchymal stem cells by alizarin red staining.)
研究者
Dr Vaishnavi Langde
Ranjeet Deshmukh Dental College and Research Centre