The Dose-response of Vitamin C-enriched Collagen on Markers of Collagen Synthesis in Healthy Young and Older Men and Women Following Resistance Exercise
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Enrollment
- 21
- Locations
- 1
- Primary Endpoint
- Change in a marker of collagen synthesis
Study Overview
Brief Summary
This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.
Detailed Description
This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.
Healthy young and older males and females ingested 0 grams, 15 grams or 30 grams HC with 50 milligrams vitamin C 1h prior to performing four sets' barbell back-squat RE at 10-repetition maximum load, after which they rested for six hours. Blood samples were collected throughout each trial to analyse procollagen type Ⅰ N-terminal propeptide (PⅠNP, a biomarker of collagen synthesis) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX, a biomarker of collagen breakdown) concentration, and the concentration of 18 amino acids that constitute collagen.
This is the first study to investigate the combined effect of different doses HC with high-intensity RE on whole body collagen synthesis in healthy young and older men and women. If 30 grams HC intake with RE does augment collagen synthesis more than RE alone, this suggests that long-term HC intake with chronic RE would be beneficial for tendon health, because collagen is the most abundant protein in tendon.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Basic Science
- Masking
- Triple (Participant, Investigator, Outcomes Assessor)
Masking Description
Before commencing each experimental trial, a laboratory technician (independent to the study) made up the three doses of hydrolyzed collagen (HC) and randomly assigned the order of HC dose (Excel 2016, Microsoft, Washington, USA) for each participant. Also, for each trial, the technician recorded the date, randomly allocated trial number (1, 2 or 3) and corresponding HC dose to blind the investigator.
Eligibility Criteria
- Ages
- 18 Years to 65 Years (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •At least 12 months experienced in resistance exercise (regular back squat experience)
- •Free from musculoskeletal injury
- •Nullipara (a woman who has never given birth) (only for young female participatns)
Exclusion Criteria
- •Having a history of patellar tendon pathology
- •Consumed nutritional supplements or medication purported to have beneficial effects on muscle-tendon properties (e.g. antioxidants, protein, etc.)
- •Had a sustained a lower limb injury in the previous six months
- •Smoker/vaper
- •<18 or >40 years old (only for young participants)
- •<40 ro >65 years old (only for older participants)
- •Having an irregular menstrual cycle (only for young female participants)
Arms & Interventions
1.Trial for young male participants: Consuming hydrolyzed collagen (HC) with reistance exercise
Young male participants consumed one of three different HC doses (0 grams, 15 grams, or 30 grams) with 4 sets of 10 repetitions of barbell back squat exercise at 10-repetition maximum load in a random order and a seven-day wash-out period interspersed between each trial.
Intervention: Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise (Dietary Supplement)
2.Trial for young female participant: Consuming hydrolyzed collagen (HC) with reistance exercise
The intervention procedure is exactly same as Arm 1 except for the number of visits. Young female participant was asked to visit the laboratory on four occasions during two consecutive months. Therefore, there were two trials in each month, where female participants' estrogen level was lower (i.e., onset of menses) or higher (i.e., ovulation). Dates for the trials were determined based on self-report of onset of menses and previous menstrual cycle length.
Intervention: Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise (Dietary Supplement)
3.Trial for older male participants: Consuming hydrolyzed collagen (HC) with reistance exercise
The age range of older male participants was 40 - 65 years. The intervention procedure is exactly same as Arm 1
Intervention: Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise (Dietary Supplement)
4.Trial for older female participants: Consuming hydrolyzed collagen (HC) with reistance exercise
The age range of older female participants was 40 - 65 years. The intervention procedure is exactly same as Arm 2
Intervention: Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise (Dietary Supplement)
Outcomes
Primary Outcomes
Change in a marker of collagen synthesis
Time Frame: at rest immediately prior to HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE
Serum procollagen type Ⅰ N-terminal propeptide (PⅠNP) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.
Change in a marker of collagen breakdown
Time Frame: at rest immediately prior to HC ingestion and 6-hour post RE
Plasma β-isomerized C-terminal telopeptide of type I collagen (β-CTX) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.
Changes in amino acids concentrations in blood
Time Frame: at rest immediately prior to HC ingestion, 0.5-hour post HC ingestion, 1-hour post HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE
Concentration of serum amino acids that constitute collagen was measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.
Secondary Outcomes
- Estrogen concentrations in women in each trial(at rest immediately prior to HC ingestion)
Investigators
Rob Erskine
Reader in Neuromuscular Physiology
Liverpool John Moores University