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Use of Empagliflozin to Treat Prediabetes

Phase 4
Recruiting
Conditions
PreDiabetes
Prediabetic State
Overweight and Obesity
Interventions
Drug: Multivitamin-Placebo
Registration Number
NCT05426525
Lead Sponsor
Oregon State University
Brief Summary

The overall purpose of this study is to identify how empagliflozin (a drug commonly used to treat type 2 diabetes) impacts skeletal muscle metabolic health among adults with prediabetes. Our aims are to: 1) Test the ability of empagliflozin to improve regulation of glucose metabolism (i.e., blood sugar) among overweight and obese individuals at risk for diabetes, and 2) Identify mechanisms to explain how empagliflozin may improve skeletal muscle glucose metabolism. We hypothesize empagliflozin will improve regulation of glucose metabolism due to changes in whole-body and skeletal muscle metabolism (e.g., increased rates of whole-body fat oxidation, evidence of impaired skeletal muscle mitochondrial respiratory function and increased energetic stress, lower accumulation of skeletal muscle lipids and improved skeletal muscle insulin signaling compared with placebo treatment).

Detailed Description

The overall study design is a 13-week, double-blind, placebo-controlled intervention trial, testing the ability of empagliflozin to improve glucose metabolism among overweight and obese individuals at risk for diabetes (compared with a multivitamin-placebo). The study involves metabolic testing before and during the intervention to identify changes in outcomes as a function of the intervention and to ensure participant safety. The study involves 9 visits to the Samaritan Athletic Medicine Center on the campus of Oregon State University in Corvallis, Oregon. Full completion of the study is anticipated to take \~4 months. The project is being completed in collaboration with physicians at Samaritan Health Services.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
40
Inclusion Criteria
  • BMI 26-45 kg/m2
  • Weight stable (± 10 lbs in previous 3 months)
  • Fasting blood glucose <126 mg/dL or HbA1c <6.5% (<48mmol/mol)
Exclusion Criteria
  • Regular moderate-vigorous exercise (≥30 min/session on ≥2 days per week)
  • Pregnancy, planning to become pregnant or nursing
  • Lidocaine allergy
  • Current or recent smoking or nicotine use (≤ 1-year abstention)
  • Medications including glucose lowering medications and supplements (SGLT2 inhibitors, GLP1 agonists, sulfonylurea, insulin, TZDs); mono-amine oxidase inhibitors; beta-blockers; diuretics
  • Major metabolic or cardiovascular conditions (e.g., type 1 diabetes, Crohn's disease, untreated hypo- or hyperthyroid, cancer, coronary artery disease, tachycardia, prior bariatric surgery, peripheral vascular disease, liver diseases (e.g., cirrhosis)
  • Diagnosed type 2 diabetes. In absence of diagnosis, two separate samples with test results of fasting blood glucose ≥126 mg/dL or HbA1c ≥6.5% (48 mmol/mol).
  • Contraindications/precautions for empagliflozin (impaired renal function (EGR<60), history of: empagliflozin hypersensitivity, ketoacidosis, hypotension, recurring urinary tract or genital mycotic infections, amputation)

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Multivitamin-PlaceboMultivitamin-PlaceboParticipants will be provided 1 multivitamin-placebo per day for 13 weeks.
EmpagliflozinEmpagliflozinParticipants will be provided 10-25mg empagliflozin per day for 13 weeks.
Primary Outcome Measures
NameTimeMethod
Insulin-stimulated glucose disposalInsulin-stimulated glucose disposal is measured before the start of the intervention (baseline) and during week 13 of the intervention.

The glucose infusion rate to maintain glycemia during insulin clamp, using plasma enrichment of glucose isotope tracer to determine changes in rates of insulin-stimulated glucose disposal

Secondary Outcome Measures
NameTimeMethod
Whole-body fat oxidationWhole-body fat oxidation is measured before the start of the intervention (baseline) and during week 13 of the intervention.

Indirect calorimetry will be used to determine the change in whole-body rate of fat oxidation during basal and insulin-stimulated conditions

Skeletal muscle insulin signalingSkeletal muscle insulin signaling is measured before the start of the intervention (baseline) and during week 13 of the intervention.

Immunoblotting to determine the change in activation of insulin signaling proteins in skeletal muscle collected at basal and during insulin-stimulated conditions

Oral glucose toleranceOral glucose tolerance is measured before the start of the intervention (baseline) and during week 12 of the intervention.

The change in blood glucose concentration in response to a 75g glucose beverage

Fasting plasma glucose concentrationFasting plasma glucose is measured before the start of the intervention (baseline) and during week 13 of the intervention.

The change in fasting plasma glucose concentration

Skeletal muscle lipidsSkeletal muscle lipids are measured before the start of the intervention (baseline) and during week 13 of the intervention.

Mass spectrometry lipidomic analysis of skeletal muscle to determine changes in muscle lipid content

Skeletal muscle mitochondrial respiratory functionSkeletal muscle mitochondrial respiratory function is measured before the start of the intervention (baseline) and during week 13 of the intervention.

Changes in skeletal muscle mitochondrial respiratory capacity measured using high-resolution respirometry

Skeletal muscle energetic stressSkeletal muscle energetic stress is measured before the start of the intervention (baseline) and during week 13 of the intervention.

Immunoblotting to determine changes in activation of AMPK and related signaling proteins pathways

Trial Locations

Locations (1)

Oregon State University

🇺🇸

Corvallis, Oregon, United States

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