Ketoconazole Contributes to Cryptorchidism Outcome Via Modulating Testicular Macrophage Trem2
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 10
- 试验地点
- 1
- 主要终点
- Relative expression of Trem2 and insulin-like 3 protein
研究概览
简要总结
Reduced hormone insulin-like 3, exclusively secreted by Leydig cells, has been identified as the recognized mechanism for cryptorchidism. Testicular macrophages residing in the testicular interstitium were thought to play a vital role in maintaining hormone secretion of Leydig cells. However, the contribution of macrophages to cryptorchidism remained poorly understood. Here, after 14 days of ketoconazole treatment, levels of both Trem2 and insulin-like 3 significantly decrease in human embryonic testes compared to the untreated control group. Conversely, 14 days of luteinizing hormone stimulation lead to elevated levels of Trem2 and insulin-like 3 in the testes compared to the untreated control group. Additionally, researchers find a positive correlation between Trem2 and insulin-like 3 expression in a human ex vivo tissue culture model. In summary, the study will propose Trem2 as a novel target for maintaining normal testicular descent by regulating the testicular immune microenvironment.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 8 Weeks 至 13 Weeks（Child）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Termination of pregnancy is not related to the health of the fetus.
- •The fetus is intact in development and free of malformations and abnormalities.
- •Estimation of gestational age based on parietal-rump length must be between 8 and 13 weeks of gestation.
排除标准
- •The termination of pregnancy is related to the health of the fetus
- •The fetus is not intact in development.
- •The fetus has malformations, abnormalities
- •Estimation of gestational age based on parietal-rump length must not be within 8-13 weeks of gestation.
研究组 & 干预措施
Intact aborted fetal testicular tissue treated with ketoconazole
A fetal developmental stage is precisely determined. Then, fetal tissues are extracted for in vitro cultivation. Intact aborted fetal testicular tissues are cultured in a medium containing 10-6 mol/L ketoconazole, which is the injectable solution powder. Ketoconazole is administered starting on the first day and then every other day, for a total of 7 times over the 14-day in vitro culture period.
干预措施: Ketoconazole (Drug)
Intact aborted fetal testicular tissue treated with luteinizing hormone
A fetal developmental stage is precisely determined. Then, fetal tissues are extracted for in vitro cultivation. Intact aborted fetal testicular tissues are cultured in a medium containing 1 IU/mL luteinizing hormone, which is the injectable solution powder. Luteinizing hormone is administered starting on the first day and then every other day, for a total of 7 times over the 14-day in vitro culture period.
干预措施: Luteinizing Hormone (Drug)
结局指标
主要结局
Relative expression of Trem2 and insulin-like 3 protein
时间窗: The date of 14 days cultured
Ketoconazole promotes a decrease in Trem2 and a decrease in insulin-like 3, and they are strongly correlated.
次要结局
未报告次要终点