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Preventive Inhalation of Hypertonic Saline in Infants With Cystic Fibrosis

Phase 2
Completed
Conditions
Cystic Fibrosis Lung Disease
Interventions
Drug: 6% Hypertonic Saline (HS), 4mL
Drug: 0.9% Isotonic Saline (IS), 4mL
Registration Number
NCT01619657
Lead Sponsor
Heidelberg University
Brief Summary

The purpose of this study is to assess whether 6% hypertonic saline (HS) is a safe and effective preventive therapy in newborns and infants with cystic fibrosis (CF).

Detailed Description

Cystic fibrosis (CF) remains one of the most common lethal genetic diseases in Europe and North America. Despite a substantial increase in life expectancy over the past decades, many CF patients still die during young adulthood due to chronic progressive CF lung disease that is caused by defective fluid transport by airway epithelia causing dehydration of airway surfaces, which in turn leads to impaired mucociliary clearance, chronic airway mucus obstruction, inflammation and infection. Recent evidence from studies in a mouse model of CF lung disease suggest that preventive improvement of airway surface hydration may be an effective treatment of early and reversible mucus obstruction and inflammation, and thus delay or ameliorate progressive damage in lungs of CF patients. Hypertonic saline (HS) is an osmotic agent that improves airway surface hydration, and inhalation of 6% HS is already an established, safe, and effective maintenance therapy that improves mucociliary clearance and lung function, and reduces pulmonary exacerbations in older children (\> 6 years) and adults with chronic CF lung disease and fixed lung damage. However, the effect of HS as a preventive therapy has not been studied, and no other therapies are available for preventive improvement of airway dehydration and mucociliary dysfunction in CF.

This investigator initiated clinical trial is a monocentric, randomized, double-blind, controlled pilot study on safety and efficacy of a preventive and early inhalation with HS in newborns and infants with CF who are diagnosed in the newborn period either by CF newborn screening (CF-NBS) or for another reason (e.g. meconium ileus) and are younger than 4 months of age at the time of enrolment. Participating patients will be randomized to 6% HS or 0.9% isotonic saline (IS) as active comparator. In both groups, patients will inhale their study solution twice daily over 52 weeks. At the beginning, during and at the end of the study, different measurements will be undertaken to determine effects of HS on safety, radiologic and/or functional alterations of the lung, number of exacerbations, time to first detection of a CF pathogen, and health-related quality of life. We expect that the results of this study will provide first evidence on the safety and efficacy of a preventive therapy that improves airway surface hydration and targets a CF basic defect and may thus delay and/or ameliorate chronic damage of the lungs of patients with CF.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
42
Inclusion Criteria
  1. Confirmed diagnosis of CF established in neonatal period either via CF newborn screening (NBS) or because of symptoms typical for CF (e.g. meconium ileus), positive family history or positive prenatal screening and fulfilling at least one of the following three criteria:

    • sweat chloride ≥ 60mEq/L
    • two CF causing mutations of CFTR gen
    • alterations of transepithelial potential difference of nasal or rectal epithelia typical for CF.
  2. Age at enrolment is 0 to 4 months.

  3. Patient's and parent's ability to comply with medication use, study visits, and study procedures is judged by the investigator (therefore parents have to understand the character of the study and individual consequences).

  4. Participation in this study is voluntary. Only patients, whose parents or legal guardians gave written consent, are included.

Exclusion Criteria
  1. Born < 30 weeks gestation.
  2. Prolonged mechanical ventilation in the first 3 months of life.
  3. A significant medical disease or condition other than CF likely to interfere with the child's ability to complete the entire protocol.
  4. Previous major surgery except for meconium ileus.
  5. Other major organ dysfunction, excluding pancreatic or hepatic dysfunction or another condition due to cystic fibrosis.
  6. Physical findings that would compromise the safety of the subject or the quality of the study data as determined by investigator.
  7. History of adverse reaction to sedation.
  8. Known hypersensitivity to study treatment.
  9. Participation in other interventional studies at the same time.

Criteria, which lead to a displacement of the procedures in sedation until the child has recovered:

  • Clinically significant upper airway obstruction as determined by investigator (e.g. severe laryngomalacia, markedly enlarged tonsils, significant snoring, diagnosed obstructive sleep apnoea).
  • Acute intercurrent respiratory infection, defined as an increase in cough, wheezing, or respiratory rate with onset in 2 weeks preceding visit.
  • Oxygen saturation <95% before initial pulmonary function test or initial MRI.
  • Severe gastroesophageal reflux, defined as persistent frequent emesis despite anti-reflux therapy.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Hypertonic Saline6% Hypertonic Saline (HS), 4mLInhalation with 6% Hypertonic Saline twice daily over 1 year
Isotonic Saline0.9% Isotonic Saline (IS), 4mLInhalation with 0.9% Isotonic Saline twice daily over 1 year
Primary Outcome Measures
NameTimeMethod
Number of patients in both treatment groups with adverse events (AEs) and serious adverse events (SAEs)during the 52 week treatment period

Safety of inhalation with HS and IS in newborns and infants with CF assessed by proportion of adverse events (AEs) and serious adverse events (SAEs)

Secondary Outcome Measures
NameTimeMethod
Rate of protocol-defined pulmonary exacerbationsduring the 52 week treatment period

Rate of protocol-defined pulmonary exacerbations requiring treatment with oral, inhaled or intravenous antibiotics between subjects randomized to HS and IS

Time to first pulmonary exacerbation in both treatment groupsduring the 52 week treatment period
Proportion of children with impairments in lung functionduring the 52 week treatment period

Proportion of children with impairments in lung function determined via multiple breath washout at baseline, after 3, 6, 9, and 12 months of inhalation in both groups

Severity of impairment in lung function testduring the 52 week treatment period

Severity of impairment in lung function test at baseline, after 3, 6, 9, and 12 months of inhalation in both groups

Health-related quality of lifeduring the 52 week treatment period

Health-related quality of life as assessed by scores from Cystic Fibrosis Questionnaire - Revised Parent Report (CFQ-R, German version), administered quarterly

Change in anthropometric and basic respiratory parametersduring the 52 week treatment period

Change in weight, height, body mass-index, weight-for-height, resting respiratory rate, and room air oxygen saturation

Proportion of patients with new isolation of CF pathogenduring the 52 week treatment period

Among participants from whom Pseudomonas aeruginosa or other CF pathogens were not isolated from respiratory cultures prior to enrolment, the proportion from whom these organisms are isolated from clinically collected respiratory cultures

Time to first isolation of a CF pathogenduring the 52 week treatment period

Time to acquisition of a CF pathogen is going to be compared between both treatment groups

Extent and severity of bronchial dilatationduring the 52 week treatment period

Extent and severity of bronchial dilatation after MRI and CXR scores at baseline and after 1 year of inhalation in both groups

Proportion of children with morphological and/or functional changes due to CF lung disease at baseline and after 1 year of inhalationduring the 52 week treatment period

Proportion of children with morphological and/or functional changes due to CF lung disease at baseline and after 1 year of inhalation according to magnetic resonance imaging (MRI) chest score and chest x-ray (CXR) Chrispin-Norman score in both groups (HS vs. IS)

Trial Locations

Locations (4)

University Hospital Gießen and Marburg GmbH

🇩🇪

Gießen, Germany

University Children's Hospital Heidelberg, Cystic Fibrosis Centre

🇩🇪

Heidelberg, Baden-Württemberg, Germany

University Children's Hospital Schleswig-Holstein

🇩🇪

Lübeck, Germany

Medizinische Hochschule Hannover

🇩🇪

Hannover, Germany

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