Study of INFLAmmasomes NLRP3 in Platelets and Leukocytes During SEPSIS in Intensive Care Unit (INFLA-SEPSIS)
试验速览
- 阶段
- 不适用
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- Activation kinetics of the NLRP3 inflammasome during sepsis until its resolution
研究概览
简要总结
septic shock is the first cause of mortality in intensive care unit. Innate immunity is the body's first line of defense against pathogens
详细描述
since their discovery, inflammasomes have an important role during inflammatory response following an aggression. There are intracytoplasmic multiprotein complex activated by cellular stress or infections and is responsible for the release of pro-inflammatory cytokines, including IL-1β. One study focused on the role of the NLRP3 inflammasome in monocyte during sepsis has shown its early alteration that is correlated with mortality. Most studies have analysed inflammasomes in nucleated cells, nevertheless, little is known about inflammasomes in platelets. The aim of the study is to analyze the activation of NLRP3 inflammasome in platelets and leukocytes during sepsis, from patients hospitalized in intensive care unit compare to a control group, until its resolution to better understand the pathophysiology of sepsis
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •hospitalized in intensive care unit with central catheter
- •age > or = 18 years
- •patients suffering from sepsis
- •hospitalized from less than72 hours
排除标准
- •patients with curatorship or guardianship
- •pregnant women
- •patients suffering from malignant blood disease
- •patients suffering from disease associated with NLRP3 inflammasome activation
- •CONTROL GROUP:
- •Inclusion Criteria:
- •hospitalized in intensive care unit with central catheter for other reason than infection
- •age > or = 18 years
- •Exclusion Criteria:
- •patients with infection disease
- •patients with curatorship or guardianship
- •pregnant women
- •patients suffering from malignant blood disease
- •patients suffering from disease associated with NLRP3 inflammasome activation
结局指标
主要结局
Activation kinetics of the NLRP3 inflammasome during sepsis until its resolution
时间窗: From day 0 to the hospital discharge, an average of 1 month
Activation kinetics of the NLRP3 inflammasome from day 0 to patient discharge measured by the area under curve
次要结局
- Severity score measured by Simplified Gravity Index 2(Day of hospital discharge, an average of 1 month)
- Rate of inflammasome NLRP3 activation(Day of hospital discharge, an average of 1 month)
- Severity score measured by Sequential Organ Failure Assessment (SOFA) score(Day of hospital discharge, an average of 1 month)