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Carotid Atherosclerosis : Innovative Imaging Biomarkers. Study Case-control

Not Applicable
Recruiting
Conditions
Carotid Stenosis
Interventions
Device: HITS quantification, Ultrasound examinations, High Resolution MRI
Registration Number
NCT02748941
Lead Sponsor
Hospices Civils de Lyon
Brief Summary

Cerebrovascular accident (CVA) constitutes a major health public problem. This represents the second cardiovascular death cause. CVA is ischemic in 80% of cases. Atheroma of large arteries, mainly carotid, is involved in about 20% of cases.

After several high grade studies (NASCET, ECST, ACAS, ACST), carotid surgery is based on stenosis calculation by Doppler ultrasound, CT angiography, MRI angiography or arteriography.

The composition of the plaque, showing its vulnerability, is associated with embolic risk and stroke. The therapeutic strategy based only on the narrowing of the arterial lumen is not satisfactory enough to prevent the occurrence of a transient ischemic attack (TIA) or an ipsilateral stroke due to carotid stenosis. Thus, new techniques emerge, to evaluate in vivo the inflammation of the plaque, its embolic consequences or the mechanical stress it undergoes. These techniques are: High Resolution MRI (HR MRI), evaluation of the Gray Scale Median (GSM) level, study of the plaque vascularization with Contrast Enhanced ultrasound (CEUS), High Intensity Transient Signals (HITS) by transcranial Doppler, micro RNA profile (mi RNA). These different modalities must be combined in order to increase the efficiency.

Based on these encouraging results, the investigator aim at evaluating the ability of different methods or their combination to predict the occurrence of ischemic stroke or TIA due to emboli from a carotid atherosclerotic plaque. Before considering a cohort study, he investigator want to evaluate the performance of each of these new methods in the characterization of carotid plaque instability. Performance evaluation will allow us to choose secondarily the most relevant association.

The investigator propose, as a first step, to make a case-control study with these methods, the cases are patients who had an ischemic stroke (authenticated by both the clinical exam and brain MRI) on the same side as the carotid stenosis and the controls are patients with carotid stenosis but without ischemic stroke. This study is a cross-sectional study because it simultaneously measures the occurrence of the event (ischemic stroke) and the characteristics of the atheromatous plaque.

Over a 2 year period, 45 symptomatic patients and 105 asymptomatic patients will be included with a carotid stenosis with at least a 50% caliber constriction according to NASCET criteria. (North American Symptomatic Carotid Endarterectomy Trial).

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
77
Inclusion Criteria
  • Carotid stenosis ≥ 50% NASCET
  • Case: ipsilateral carotid stenosis stroke, certified by MRI or CT in the preceding month, with no others causes of stroke
  • Control: no clinical sign or MRI hint for a recent stroke
  • Signed informed consent form
  • Patient affiliated to a social security system or equivalent
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Exclusion Criteria
  • Modified Rankin scale > 3
  • Homolateral stroke or TIA > 1 month
  • Medical history of homolateral carotid surgery, cervical radiation, carotid occlusive, homolateral intracranial stenosis, risk of developing arterial thromboembolic events
  • Renal failure (creatinine clearance < 50 ml/min)
  • Contraindication to ultrasound (sonovue)
  • Contraindication to MRI and its contrast media (Gadolinium)
  • Inability to sign informed consent
  • Inability to undergo any of the technics (claustrophobia...)
  • Serious co-morbid disease, dementia
  • Neurological disease with no carotid disease
  • Risk of pregnancy or pregnancy or breastfeeding
  • Adult under reinforced guardianship or legal guardian
  • Patient not understanding French
Read More

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
symptomatic and asymptomatic patientsHITS quantification, Ultrasound examinations, High Resolution MRI-
Primary Outcome Measures
NameTimeMethod
ulceration or cap rupture présence in HR MRIDay 1
Number of HITS per hour in doppler ultrasonographyDay 1
GSM (Gray Scale Median) quantification in doppler ultrasonographyDay 1
Ulceration > 2 mm presence in doppler ultrasonographyDay 1
hemorrhage intra-plaque présence in HR MRIDay 1
large lipid-rich necrotic core présence in HR MRIDay 1
CEUS (Contrast Enhanced UltraSound )Day 1

signal intensity amplification quantification and Ulceration \> 2 mm

Secondary Outcome Measures
NameTimeMethod
shear stresses (Pa) on the surface of the plaqueDay 1

Quantification of the shear stresses (Pa) on the surface of the plaque with an analysis of hemodynamic environment using fluid dynamics mathematical models based on data collected by MRI.

mi-RNA identificationDay 1

Identification of circulating mi-RNA specific to unstable atherosclerotic plaque by RT-PCR and comparison between case and control

large lipid-rich necrotic coreDay 1

Compare the results of each modality for pathological analysis of the surgical equipment.

ulceration or cap ruptureDay 1

Compare the results of each modality for pathological analysis of the surgical equipment.

hemorrhage intra-plaqueDay 1

Compare the results of each modality for pathological analysis of the surgical equipment.

Trial Locations

Locations (1)

Hospices Civils de Lyon - Service de radiologie

🇫🇷

Bron, France

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