Diagnosis of Periodontal Disease Using Genetic Marker in Saliva
Not Applicable
- Conditions
- Chronic Periodontitis
- Interventions
- Procedure: non-surgical periodontal therapy
- Registration Number
- NCT02069574
- Lead Sponsor
- Asan Medical Center
- Brief Summary
The purpose of the present study is to identify the novel periodontal disease biomarkers using differentially expressed microRNA in the saliva. The severity of chronic periodontal diseases and the time of pre- and post-treatment are major classification criteria. The obtained data will secure the foundation for the development of a new non-invasive diagnostic methods for early diagnosis \& prognosis prediction of periodontal disease.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 18
Inclusion Criteria
A. Severe Periodontal Disease
- Patients with chronic severe periodontitis
- Primary treatment of periodontal scaling, subgingival curettage is required, and the general progress of the protocol agreed with the basic periodontal therapy patients.
- subjects agreed collecting saliva and gingival crevicular fluid and physicochemical tests and genetic testing
A. Moderate Periodontal Disease
- Patients with chronic moderate periodontitis
- Primary treatment of periodontal scaling, subgingival curettage is required, and the general progress of the protocol agreed with the basic periodontal therapy patients.
- subjects agreed collecting saliva and gingival crevicular fluid and physicochemical tests and genetic testing
C. Healthy Control
- Healthy periodontal patients ( more than 20 permanent teeth are present , in all parts of deep periodontal pockets measuring 4mm or less, and height is 2mm or less clinical attachment person)
- subjects agreed collecting saliva and gingival crevicular fluid and physicochemical tests and genetic testing
Exclusion Criteria
- Received periodontal treatment within the last six months
- recently taken antibiotics within 3 months if you need a prescription antibiotic prophylaxis
- taking the drug can affect periodontal status for a long period of time
- pregnant or breastfeeding
- In case of oral inflammatory mucosal disease (Lichen planus, etc.)
- A history of alcoholism or drug abuse if
- other special systemic disease (high blood pressure)
- ages under 18
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description severe periodontitis group non-surgical periodontal therapy non-surgical periodontal therapy Moderate periodontitis group non-surgical periodontal therapy non-surgical periodontal therapy
- Primary Outcome Measures
Name Time Method Expression level of MicroRNAs using Microarray Change from Baseline in expression level of MicroRNAs at 1,6,12 months
- Secondary Outcome Measures
Name Time Method Expression level of cytokines using multiple cytokine analysis Change from Baseline in expression level of cytokines at 1,6,12 months * Pro-inflammatory biomarker : interleukin(IL)-1a, IL-1b, IL-6, IL-8
* Matrix metalloproteinase(MMP) : MMP-8, MMP-9
* Anti-inflammatory biomarker: IL-10, inhibitor of MMP (TIMP)-1
Trial Locations
- Locations (1)
ASAN Medical Center
🇰🇷Seoul, Korea, Republic of