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A 12-weeks Study to Evaluate Sulforaphane in Treatment of Autism Spectrum Disorder

Not Applicable
Completed
Conditions
Autism Spectrum Disorder
Interventions
Other: Placebo
Dietary Supplement: Sulforaphane
Registration Number
NCT02879110
Lead Sponsor
Central South University
Brief Summary

In this proposed study, the investigators will evaluate the the efficacy, safety and related mechanism of sulforaphane in treatment of autism spectrum disorder (ASD). The study will recruit 120 ASD patients, then these patients will be randomized to sulforaphane group or placebo group (60 patients per arm) for 12 weeks clinic trial. Clinical efficacy and safety assessment will be done at screen/baseline, 4 week, 8 week and 12 week. The specific aims are to compare sulforaphane versus placebo on: 1) clinical core symptoms; 2) other behavioral problems and adaptive behaviors. Biological samples also will be collected, and stored to research related mechanisms.

Detailed Description

In this proposed study, the investigators will evaluate the the efficacy, safety and related mechanism of sulforaphane in treatment of autism spectrum disorder (ASD). The study will recruit 120 ASD patients, then these patients will be randomized to sulforaphane group or placebo group (60 patients per arm) for 12 weeks clinic trial. Clinical efficacy and safety assessment will be done at screen/baseline, 4 week, 8 week and 12 week. The specific aims are to compare sulforaphane versus placebo on: 1) clinical core symptoms; 2) other behavioral problems and adaptive behaviors. The investigators hypothesize that (1) sulforaphane is superior to placebo in the treatment of clinical symptoms in patients with ASD, measured by the Social Responsiveness Scale, Aberrant Behavior Checklist, Repetitive Behavior Scale - Revised and Ohio State University Autism Clinical Global Impression; (2) sulforaphane is superior to placebo in the treatment of other behavioral problems and adaptive behaviors patients with ASD, measured by Achenbach's Child Behavior Checklist and Adaptive Behavior Assessment System, Second Edition; and (3) Biological samples will be collected, and stored so that the hypothesis sulforaphane may alter oxidative stress indexes or inflammatory biomarkers, and influence histone deacetylase inhibitor mechanism or inflammatory mechanism et al that may be significantly correlated with clinical improvement.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
110
Inclusion Criteria
  1. Aged 3 to 15 years.
  2. Meet DSM-V diagnostic criteria for autism spectrum disorder, and been checked with Autism Diagnostic Interview-Revised (ADI-R) and Autism Diagnostic Observation Schedule (ADOS).
Exclusion Criteria
  1. With severe physical disease (i.e. congenital heart disease, thyroid disease, diseases with severe abnormality of liver or kidney function, diseases with abnormality vision or hearing et al.)
  2. With severe central nervous system disease (i.e. epilepsy et al).
  3. With other specific genetic syndromes (i.e. Fragile-X syndrome, Down's syndrome et al.)

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Placebo groupPlaceboThe patients will take placebo for 12 weeks.
Sulforaphane groupSulforaphaneThe patients will take sulforaphane for 12 weeks.
Primary Outcome Measures
NameTimeMethod
The change of social impairments of children with autism spectrum disorderAt baseline, 4 week, 8 week and 12 week/endpoint

Social impairments are measured by Social Responsiveness Scale

Secondary Outcome Measures
NameTimeMethod
The change of other behavioral problems of children with autism spectrumAt baseline, 4 week, 8 week and 12 week/endpoint

Other behavioral problems are measured by Achenbach's Child Behavior Checklist

The change of height as measured by Height measurement toolsAt baseline and 12 week/endpoint
The change of blood lipid as tested by clinical laboratoryAt baseline and 12 week/endpoint
The change of clinical symptoms of children with autism spectrumAt baseline, 4 week, 8 week and 12 week/endpoint

Clinical symptoms are measured by Aberrant Behavior Checklist

The change of HBV test as tested by clinical laboratoryAt baseline and 12 week/endpoint
The change of urine routine test as tested by clinical laboratoryAt baseline and 12 week/endpoint
The change of rigid interests and behaviors of children with autism spectrum disorderAt baseline, 4 week, 8 week and 12 week/endpoint

Rigid interests and behaviors are measured by Repetitive Behavior Scale - Revised

The change of clinical general impression of children with autism spectrumAt baseline, 4 week, 8 week and 12 week/endpoint

Clinical general impression is measured by Ohio State University Autism Clinical Global Impression

The change of blood routine test as tested by clinical laboratoryAt baseline and 12 week/endpoint
The change of fasting blood-glucose as tested by clinical laboratoryAt baseline and 12 week/endpoint
The change of kidney function as tested by clinical laboratoryAt baseline and 12 week/endpoint
The change of heart rate as measured by stopwatchAt baseline and 12 week/endpoint
The change of liver function as tested by clinical laboratoryAt baseline and 12 week/endpoint
The change of adaptive behaviors of children with autism spectrumAt baseline, 4 week, 8 week and 12 week/endpoint

Adaptive behaviors are measured by Adaptive Behavior Assessment System, Second Edition

The change of weight as measured by weighing-machineAt baseline and 12 week/endpoint
The change of thyroid function as tested by clinical laboratoryAt baseline and 12 week/endpoint
The change of helicobacter pylori test as tested by clinical laboratoryAt baseline and 12 week/endpoint
Number of participants with treatment-related adverse events as assessed by Systematic Assessment for Treatment Emergent EffectsAt 4 week, 8 week and 12 week/endpoint

Trial Locations

Locations (2)

Guangzhou Huiai Hospital

🇨🇳

Guangzhou, Guangdong, China

The second Xiangya hospital of central south university

🇨🇳

Changsha, Hunan, China

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