Probing the Salivary Proteome for Prognostic Biomarkers in Response to Non-Surgical Management of Periodontitis
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Enrollment
- 10
- Locations
- 1
- Primary Endpoint
- Saliva protein content change
Study Overview
Brief Summary
This study aims to investigate salivary proteome changes in periodontitis patients before and after non-surgical treatment. Ten systemically healthy and non-smoker individuals with stage III, grade C periodontitis underwent non-surgical periodontal treatment. Saliva was collected at baseline, and one and six months post-treatment. Whole-mouth plaque and gingival index, probing depth, bleeding on probing and clinical attachment loss were measured. The saliva proteome was investigated by label-free quantitative proteomics. Normalized protein intensities were measured and protein changes were modeled over time with significant protein regulation considered at false discovery rate (FDR)<0.05.
Detailed Description
Study Population and Clinical Examination
A total of 10 systemically healthy and non-smoker individuals with stage III, grade C periodontitis were recruited at the Department of Periodontology, School of Dentistry, Aydın Adnan Menderes University, Aydın, Turkey.
Full-mouth periodontal assessment included measurements of probing depth, clinical attachment loss, the percentage of sites with bleeding on probing, gingival index, and plaque index at baseline (T0) and one month (T1) and six months (T6) post-treatment. All measurements were obtained from 6 sites per tooth excluding third molars using a manual periodontal probe (William's periodontal probe, Hu-Friedy, Chicago, IL). The alveolar bone resorption was assessed on the digital panoramic radiograph in each participant. All clinical measurements were performed by an experienced periodontist (B.A.).
Diagnosis of stage III, grade C periodontitis was performed according to the criteria proposed by the 2017 International Workshop on the Classification of Periodontal and Peri-implant Diseases and Conditions.
Treatment
Study Design
- Study Type
- Observational
- Observational Model
- Case Only
- Time Perspective
- Prospective
Eligibility Criteria
- Ages
- 32 Years to 43 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Non-smokers
- •Having at least 20 natural teeth.
Exclusion Criteria
- •Having any chronic inflammatory or immunological conditions such as diabetes mellitus, cardiovascular diseases, rheumatoid arthritis and mucocutaneous diseases,
- •Pregnant or lactating
- •Intake of antimicrobials, anti-inflammatory drugs and immunosuppressive agents within the last 6 months
- •Non-surgical/surgical periodontal therapy within 12 months
- •Having orthodontic appliances, removable partial dentures and restorative and endodontic treatment requirements.
Outcomes
Primary Outcomes
Saliva protein content change
Time Frame: Change from baseline to 1 month and 6 months after treatment
pg/ml
Secondary Outcomes
No secondary outcomes reported
Investigators
Beral Afacan
Principal Investigator
Aydin Adnan Menderes University