Methodology Antiseptic Application, Influence on Oral Biofilm.
- Conditions
- Oral BiofilmPeriodontitisGingivitis
- Interventions
- Drug: essential oils, immersionDrug: Chlorhexidine, immersionDrug: chlorhexidine, mouthwashDrug: essential oils, mouthwashProcedure: professional oral cleaningProcedure: Plaster castDevice: IDODS
- Registration Number
- NCT02267239
- Lead Sponsor
- University of Santiago de Compostela
- Brief Summary
The study has the objective to compare two different methodology of application the antiseptic on the oral biofilm will be formed during 48h in situ on a specific device. The first one using the immersion in the antiseptic solution, as much of the authors did in the past. And the second way doing a active mouthwash, as the manufacturer recommends.
The study will compare two different antiseptic solutions, the chlorhexidine (0.2% Oraldine Perio) and the essential oils (Listerine Mentol) with the two different application and compared the bacterial vitality, thickness and covering grade between solutions and with a basal sample.
- Detailed Description
15 healthy volunteers, between 20 and 45 years old, will use during 48 hours two specific oral devices with 3 glass disks each. After this period, one of the disk will be drawn from the device and it will be immersed in Live/Dead BacLight solution for 15 minutes and then the disk will be analysed by the Confocal Laser Scanning Microscope (CLSM). Immediately, another disk will be removed from the intraoral device and immersed in the antiseptic solution, being analysed by the CLSM as well. After that, the volunteers will do the mouthwash with the antiseptic solution (as stated in the specific protocol) and the last disk will be drawn from the device and will be analysed by the CLSM following the same protocol. The other 3 disk will undergo the same protocol of baseline, immersion and mouthwash, but with the other antiseptic solution.
The CLSM data will be analysed and the bacterial vitality, thickness and covering grade will be calculated. Finally the statistical analysis will be applied (Pairwise comparisons, with the Bonferroni adjustment).
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 15
- good oral health status: a minimum of 24 permanent teeth with no evidence of gingivitis or periodontitis and absence of untreated caries
- smoker or former smoker
- presence of dental prostheses or orthodontic devices
- antibiotic treatment or routine use of oral antiseptics in the previous three months
- presence of any systemic disease that could alter the production or composition of saliva
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Essential Oils, immersion essential oils, immersion Professional oral cleaning Plaster cast IDODS Immersion the disk with the biofilm in the essential oils antiseptic solution Essential Oils, immersion IDODS Professional oral cleaning Plaster cast IDODS Immersion the disk with the biofilm in the essential oils antiseptic solution Chlorhexidine, immersion Chlorhexidine, immersion Professional oral cleaning Plaster cast IDODS Immersion the disk with the biofilm in the chlorhexidine antiseptic solution Chlorhexidine, immersion professional oral cleaning Professional oral cleaning Plaster cast IDODS Immersion the disk with the biofilm in the chlorhexidine antiseptic solution Chlorhexidine, immersion IDODS Professional oral cleaning Plaster cast IDODS Immersion the disk with the biofilm in the chlorhexidine antiseptic solution baseline Plaster cast Professional oral cleaning Plaster cast IDODS Disk in basal conditions. Chlorhexidine, mouthwash chlorhexidine, mouthwash Professional oral cleaning Plaster cast IDODS Active mouthwash with the chlorhexidine antiseptic solution Chlorhexidine, mouthwash professional oral cleaning Professional oral cleaning Plaster cast IDODS Active mouthwash with the chlorhexidine antiseptic solution Essential Oils, mouthwash essential oils, mouthwash Professional oral cleaning Plaster cast IDODS Active mouthwash with the essential oils antiseptic solution Chlorhexidine, mouthwash Plaster cast Professional oral cleaning Plaster cast IDODS Active mouthwash with the chlorhexidine antiseptic solution Essential Oils, immersion professional oral cleaning Professional oral cleaning Plaster cast IDODS Immersion the disk with the biofilm in the essential oils antiseptic solution baseline professional oral cleaning Professional oral cleaning Plaster cast IDODS Disk in basal conditions. baseline IDODS Professional oral cleaning Plaster cast IDODS Disk in basal conditions. Essential Oils, mouthwash Plaster cast Professional oral cleaning Plaster cast IDODS Active mouthwash with the essential oils antiseptic solution Essential Oils, mouthwash professional oral cleaning Professional oral cleaning Plaster cast IDODS Active mouthwash with the essential oils antiseptic solution Essential Oils, mouthwash IDODS Professional oral cleaning Plaster cast IDODS Active mouthwash with the essential oils antiseptic solution Chlorhexidine, mouthwash IDODS Professional oral cleaning Plaster cast IDODS Active mouthwash with the chlorhexidine antiseptic solution Essential Oils, immersion Plaster cast Professional oral cleaning Plaster cast IDODS Immersion the disk with the biofilm in the essential oils antiseptic solution Chlorhexidine, immersion Plaster cast Professional oral cleaning Plaster cast IDODS Immersion the disk with the biofilm in the chlorhexidine antiseptic solution
- Primary Outcome Measures
Name Time Method change in bacterial vitality baseline, 30seconds after immersion and 30 seconds after mouthwash (in both antiseptic solutions) percentage of live bacteria in the sample
change in thickness baseline, 30 seconds after immersion and 30 seconds after mouthwash (in both antiseptic solutions) maximum numbers of layers (1micra per layer) with continuous presence of bacteria
change in covering grade baseline, 30seconds after immersion and 30 seconds after mouthwash (in both antiseptic solutions) percentage of the disk surface occupied by the biofilm
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (1)
University of Santiago de Compostela
đŸ‡ªđŸ‡¸Santiago de Compostela, A Coruña, Spain