Metabolic Impact of the Digestion of Fat in Emulsified vs Non-emulsified Form in Lean or Obese Volunteers
试验速览
- 阶段
- 不适用
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- Time of appearance of plasma triglyceride peak after ingestion of emulsified vs unemulsified fat during breakfast
研究概览
简要总结
In this study, the investigators are interested in investigating how the emulsification state and amount of fat in a meal can modify the kinetics of postprandial lipemia and inflammatory outcomes (including endotoxemia) in obese vs lean subjects.
10 lean and 10 obese volunteers will come 3 times (>3 weeks apart) at the investigation center to receive a standard breakfast containing milk fat (10 g non emulsified, or 40 g non emulsified, or 40 g finely emulsified). 13C triglyceride stable isotopes will allow to follow the metabolic fate of fatty acids and calculate lipid oxidation by breath test. Blood sampling throughout digestion will allow to measure metabolic, lipid and inflammation parameters. Stool will be analysed to determine lipid losses in faeces and to phenotype microbiota.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •No smokers
- •BMI 18 to 35 kg/m2
- •Moderate physical activity
- •Safety subject during medical consultation
排除标准
- •Medical history which may affect lipid metabolism and gut microflora (renal -cardiovascular -hepatic- endocrine-inflammatory diseases)
- •Drug use that could affect lipid metabolism and gut microflora (steroids, antilipemic agent, anorectic, antibiotic)
- •Inflammatory or infectious disease in the last three month
- •C Reactive Protein > 20
- •Prebiotic or probiotic high consumers (several times a day)
- •Eating disorder
- •Claustrophobic subjects
研究组 & 干预措施
10 g non emulsified of milk fat
They are compared using the repeated measurements taken within subjects
干预措施: All subjects receive three sequences of a standard breakfast (Other)
40 g non emulsified of milk fat
The subjects receive three sequences of different breakfasts. They are compared using the repeated measurements taken within subjects.
干预措施: All subjects receive three sequences of a standard breakfast (Other)
40 g finely emulsified of milk fat
The subjects receive three sequences of different breakfasts. They are compared using the repeated measurements taken within subjects.
干预措施: All subjects receive three sequences of a standard breakfast (Other)
结局指标
主要结局
Time of appearance of plasma triglyceride peak after ingestion of emulsified vs unemulsified fat during breakfast
时间窗: four hours
次要结局
- Kinectics of: Lipid oxidation plasma metabolite concentrations Endotoxemia and plasma markers of inflammation 13 C-fatty acid appearance in plasma(one day)