A Prospective Study Comparing the Outcomes of Single Embryo Culture Versus Group Culture in Preimplantation Genetic Testing Cycles
Trial Snapshot
- Phase
- Not Applicable
- Sponsor
- Enrollment
- 236
- Locations
- 1
- Primary Endpoint
- high quality blastocyst formation rate
Study Overview
Brief Summary
The aim of this study is to analyze whether there is difference in the effect of single embryo culture and group culture in preimplantation genetic screening (PGS) cycle, and provide reference for the feasibility of the clinical application of medium-based noninvasive PGD. This prospective study prepares for more than 1,000 normal fertilized zygotes in each group over one year period. For the recruited patients, all normal fertilized zygotes were randomly allocated into two groups: cultured individually in 25 ul media or 2-3 embryos cultured together in 50 ul media. The blastocyst formation rates, aneuploid rates, clinical pregnancy rates, implantation rates and miscarriage rates will be compared between the two groups.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Other
- Masking
- None
Eligibility Criteria
- Ages
- 20 Years to 45 Years (Adult)
- Sex
- Female
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •patients treated by preimplantation genetic diagnostic / screening cycles
Exclusion Criteria
- •number of normal fertilization zygotes is less than 6
Arms & Interventions
Single embryo culture
Embryo is cultured individually in 25 ul media
Intervention: embryo culture (Other)
Group embryo culture
2-3 Embryos are group cultured in 50 ul media
Intervention: embryo culture (Other)
Outcomes
Primary Outcomes
high quality blastocyst formation rate
Time Frame: 6 days after fertilization
number of blastocysts with high quality divided by number of zygotes with normal fertilization
Secondary Outcomes
- embryo euploid rate(about one month after blastocyst biopsy)
- embryo implantation rate(about three to six months after blastocyst biopsy)