A Prospective Study Comparing the Outcomes of Single Embryo Culture Versus Group Culture in Preimplantation Genetic Testing Cycles
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 236
- 试验地点
- 1
- 主要终点
- high quality blastocyst formation rate
研究概览
简要总结
The aim of this study is to analyze whether there is difference in the effect of single embryo culture and group culture in preimplantation genetic screening (PGS) cycle, and provide reference for the feasibility of the clinical application of medium-based noninvasive PGD. This prospective study prepares for more than 1,000 normal fertilized zygotes in each group over one year period. For the recruited patients, all normal fertilized zygotes were randomly allocated into two groups: cultured individually in 25 ul media or 2-3 embryos cultured together in 50 ul media. The blastocyst formation rates, aneuploid rates, clinical pregnancy rates, implantation rates and miscarriage rates will be compared between the two groups.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 45 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •patients treated by preimplantation genetic diagnostic / screening cycles
排除标准
- •number of normal fertilization zygotes is less than 6
研究组 & 干预措施
Single embryo culture
Embryo is cultured individually in 25 ul media
干预措施: embryo culture (Other)
Group embryo culture
2-3 Embryos are group cultured in 50 ul media
干预措施: embryo culture (Other)
结局指标
主要结局
high quality blastocyst formation rate
时间窗: 6 days after fertilization
number of blastocysts with high quality divided by number of zygotes with normal fertilization
次要结局
- embryo euploid rate(about one month after blastocyst biopsy)
- embryo implantation rate(about three to six months after blastocyst biopsy)