A Phase I Clinical Trial to Evaluate the Safety and Tolerability of Allogeneic Bone Marrow-derived Mesenchymal Stem Cells in Patients With Severe Anti-aqua Porin-4-immunoglobulin G Positive Neuromyelitis Optica Spectrum Disorder Relapse
试验速览
- 阶段
- 1 期
- 状态
- 尚未招募
- 入组人数
- 6
- 主要终点
- Dose-Limiting Toxicity
研究概览
简要总结
To evaluate the safety and tolerability of allogeneic bone marrow-derived mesenchymal stem cells (CE211NS21) in patients with severe anti-aquaporin-4-immunoglobulin G positive neuromyelitis optica spectrum disorder (AQP4-IgG-positive NMOSD) relapse
详细描述
CE211NS21 is an allogeneic myeloid-derived mesenchymal stem cell therapy that secretes various growth factors and cytokines and regulates immune activity to rejuvenate myelin, and this clinical trial aims to confirm the safety and tolerability of CE211NS21 in phase 1 clinical trials in patients with anti-aquaporin4 antibody-positive optic spectrum category disease.
In terms of risk assessment, the safety was confirmed when 1x10^6 cells/kg of CE211NS21 was administered to C57BL6 mice three times at intervals of two weeks, and safety and tolerability were confirmed when 1x10^6 cells/kg of allogeneic myeloid-derived mesenchymal stem cells (CS20BR08) were administered in the intrathecal twice at 28 days intervals in therapeutic use in NMOSD patients.
When CE211NS21 was administered to C57BL6 mice intrathecal, the lethal dose exceeded the high dose of 1x10^6 cells/kg, and the non-toxic dose (NOAEL) was 1x10^6 cells/head/20uL when administered repeatedly three times at intervals of two weeks into the intrathecal. Therefore, in this clinical trial, CE211NS21 (step 1 dose 1.0x10^6 cells/kg, step 2x10^6 cells/kg) was set to be administered three times in consideration of the toxicity test result of three repeated doses and the safety of up to three doses in therapeutic clinical trials.
And the administration administers the first or second-stage dose into the intrathecal at baseline, at 4 weeks and at 16 weeks.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 19 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •1. Male or female adults aged 19 years or older and 65 years or younger (by full age) at the time of obtaining informed consent.
- •2. Patients who have a bone marrow donor who is 19 years or older and 70 years or younger (by full age) among their blood relatives.
- •3. Patients diagnosed with anti-aquaporin-4-immunoglobulin G (AQP4-IgG) positive neuromyelitis optica spectrum disorder based on serum testing at the time of screening.
- •4. Patients who have experienced a severe relapse† (first attack or acute relapse) within 28 days prior to screening and meet the following criteria. However, in patients with both transverse myelitis and optic neuritis, the criteria for the site of relapse are applied.
- •\ Expanded disability status scale (EDSS) scale of 4.0 or higher and 8.5 or lower.
- •\ Best corrected visual acuity (BCVA) of 20/200 or lower in one or both eyes.
- •†Relapse: New onset of neurological symptoms related to neuromyelitis optica or worsening of pre-existing neurological symptoms, objective changes on neurological examination (clinical findings or MRI findings) persisting for 24 hours or more, or the new onset of neurological symptoms or worsening of neurological symptoms requiring treatment.
- •5. Patients who can come to outpatient visits alone or with caregiver assistance
- •6. Women of childbearing potential who have not undergone sterilization must agree to use appropriate contraception\* until 12 months after the administration of the investigational product, and must provide evidence of not being in a childbearing state at the time of screening by meeting one of the following criteria:
- •7. For male patients who have not undergone a vasectomy: The patient must agree to use a barrier method of contraception (e.g., condoms) and ensure that he and his partner use appropriate contraception\* until 12 months after the administration of the investigational product, and must agree to refrain from donating sperm.
- •8. Patients who have been fully informed about this clinical trial, have voluntarily agreed to participate, and have given written consent to comply with the clinical trial's requirements.
排除标准
- •1. Patients with any of the following cardiovascular diseases at the time of screening:
- •* Myocardial infarction, unstable arrhythmia, and/or unstable angina within 6 months.
- •* QTc interval ≥ 450 milliseconds or clinically significant changes on the electrocardiogram.
- •* Congestive heart failure of New York Heart Association (NYHA) Class II or higher.
- •* Stroke or transient ischemic attack (TIA) within 6 months.
- •2. Patients with a history of any other malignancy within 5 years prior to screening.
- •3. Patients who are HIV positive.
- •4. Patients deemed unsuitable for participation in this clinical trial by the investigator based on active hepatitis (HBV, HCV) test results.
- •5. Patients with acute or severe infections.
- •6. Patients for whom lumber puncture is contraindicated.
- •7. Patients with a history of major neurological diseases other than the target disease (including a history of polio).
- •8. Patients suspected of having demyelinating diseases other than the target disease or progressive multifocal leukoencephalopathy (PML).
- •9. Patients with a history of active infection within 4 weeks prior to screening (patients with conditions such as onychomycosis or dental caries may be allowed to participate in this clinical trial if deemed suitable by the investigator).
- •10. Patients who have undergone major surgery requiring general anesthesia within 4 weeks prior to screening.
- •11. Patients who are legally incapacitated, have active psychiatric disorders, or have severe neurological or psychiatric problems that could affect the conduct of the clinical trial.
- •12. Patients who have previously received other cell therapies.
- •13. Patients with hypersensitivity to bovine proteins, penicillin, or streptomycin antibiotics.
- •14. Patients with a history of hypersensitivity to any components of the investigational product.
- •\ 15. Patients who require high-dose steroids exceeding 0.5 mg/kg/day or pulse steroid therapy at the baseline\*.
- •16. Patients who have received immunosuppressants or immunomodulators other than concomitantly allowable immunosuppressive therapy (IST), or any other investigational products within 12 weeks prior to screening (or within 5 times the medication's half-life).
- •\ 17. Patients whose laboratory test results at screening fall into any of the following categories:
- •* ANC \<1,500/mm³
- •* Platelet count \<100,000/mm³
- •* Hemoglobin \<9.0 g/dL (Patients may be eligible if hemoglobin level recovers to 9.0 g/dL or higher. However, transfusions within 7 days prior to screening to meet this criterion are not allowed.)
- •* Serum creatinine \>2.0xUNL
- •* Total bilirubin \>2.0xUNL
- •* AST, ALT \>3.0xUNL \ 18. Pregnant or breastfeeding women, or those with a positive pregnancy test at screening.
- •19. Patients who have been enrolled in another clinical trial within 12 weeks prior to screening (however, participation is allowed for those enrolled in non-interventional observational studies without the administration of investigational products).
- •20. Patients deemed unsuitable for participation in this clinical trial by the investigator for any other reasons.
研究组 & 干预措施
CE211NS21
baseline (D0), 4-week point (Visit 3), and 16-week point (Visit 6) for intrathecal administration of CE211NS21.
干预措施: CE211NS21 (Biological)
结局指标
主要结局
Dose-Limiting Toxicity
时间窗: [Time Frame: up to 4 weeks]
To evaluate the incidence of dose-limiting toxicity (DLT) of CE211NS21 Injection in patients with NMOSD relapse.
Adverse Events (AEs)
时间窗: [Time Frame: up to 4 weeks]
To evaluate the safety and tolerability of CE211NS21 Injection in patients with NMOSD relapse by assessing treatment-emergent adverse events.
次要结局
- 36-item Short Form Survey (SF-36)([Time Frame: 12 weeks, 24 weeks])
- Functional System Score(12 weeks, 24 weeks)
- Expanded Disability Status Scale (EDSS)([Time Frame: 12 weeks, 24 weeks])