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Transplantation of Autologous Olfactory Ensheathing Cells in Complete Human Spinal Cord Injury

Phase 1
Conditions
Complete Spinal Cord Injuries
Interventions
Procedure: olfactory mucosa ensheathing cell grafting, rehabilitation
Other: rehabilitation
Registration Number
NCT01231893
Lead Sponsor
Wroclaw Medical University
Brief Summary

The purpose of this experimental therapy is an assessment of the safety and feasibility of transplantation of autologous olfactory ensheathing glia and olfactory fibroblasts obtained from the olfactory mucosa in patients with complete spinal cord injury.

Detailed Description

The increasing number of patients (mostly young), who have sustained a spinal cord injury mainly as a result of motor vehicle accidents, falls or violence has become worldwide a serious clinical, social and economical problem. Most accepted treatment protocols for spinal cord injury focus on techniques of early neuro-protection aimed at maximal prevention of secondary spinal cord injury (administration of methylprednisolon and spinal cord surgical decompression) as well as on methods of stimulation of plasticity in the central nervous system (neurorehabilitation). While these methods have been shown to stimulate functional recovery in patients with incomplete spinal cord injury, the results of treatment of patients with severe incomplete and complete spinal cord injuries remain unsatisfactory. This is due to the lack of spontaneous regeneration of lesioned axons in the spinal cord. Results from a substantial number of animal experiments performed mainly on the model of mammalian spinal cord injury in the last 3 decades led to the establishment of numerous regeneration-promoting strategies including application of neurotrophic factors, antibodies blocking the myelin-associated proteins and transplantation of cells with neurotrophic activity. Olfactory ensheathing cells (OECs) are an unique population of macroglia found in the lamina propria of olfactory mucosa, around the olfactory nerve fascicles and in the two outer layers of the olfactory bulb. These cells have the natural ability to stimulate the regrowth of lesioned peripheral and central axons. In a Phase I non-randomized controlled prospective study we have tested the hypothesis that a combined approach for treatment of complete spinal cord injuries consisting of intraspinal transplantation of a mixture of autologous OECs and fibroblasts isolated from the olfactory mucosa, combined with intense neuro-rehabilitation is safe and feasible.

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
10
Inclusion Criteria
  • spinal cord injury at subacute or chronic stage
  • a single spinal cord injury between segments C5 and L5
  • myelopathy confirmed by MRI, not exceeding 2 spinal cord segments
  • loss of sensory and motor function below the injury, confirmed in control studies (ASIA Category A)
  • age from 16 to 65 years
  • patient undergoing continuous rehabilitation
  • good patient motivation and cooperation
  • signed informed consent
Exclusion Criteria
  • a coexisting lesion of the nervous system
  • progressive post-traumatic syringomyelia
  • significant spinal stenosis or instability
  • persistent neuropathic pain
  • muscle atrophy or joint ossifications
  • severe systemic disease (neoplasm, contagious disease, diabetes etc.)
  • chronic sinusitis
  • tumors or polyps of nasal cavities
  • persistent hyposmia or anosmia
  • pregnant or breastfeeding women

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
olfactory ensheathing cell recipientolfactory mucosa ensheathing cell grafting, rehabilitation-
controlrehabilitation-
Primary Outcome Measures
NameTimeMethod
Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Department of Neurosurgery of Wroclaw Medical University

🇵🇱

Wroclaw, Poland

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