GCF Levels of IL-1β and IL-6 in Gingivitis and Stage III-Grade C Periodontitis

Completed

• Conditions: Periodontitis
• Interventions: Diagnostic Test: gingival crevicular fluid

• Registration Number: NCT04689438
• Lead Sponsor: Uşak University

Brief Summary

Periodontal diseases are chronic diseases that occur as a result of a violation of the balance between microbial dental plaque and the host response. Gingivitis is a disease characterized by inflammation of the gingiva that occurs in one or more areas without loss of attachments.1 in periodontitis, an inflammatory event that begins in the gingiva along with gingivitis spreads to the periodontal ligament, alveolar bone and soft tissues that support the tooth, causing the destruction of these structures.2 Cytokines are low molecular weight proteins that participate in the initial and active stages of inflammation and immunity. In periodontal disease pathogenesis, cytokine response has been reported to play a very critical role in determining disease progression.3 IL-1beta and IL-6 are key cytokines in chronic inflammatory diseases and have the potential to initiate bone loss and tissue destruction seen in periodontal disease.4the purpose of this study; it is to determine the degree of inflammation and periodontal destruction by determining the levels of IL-1beta and IL-6 cytokines in the gingival crevicular fluid of periodontal healthy and diseased individuals.

Detailed Description

Periodontal measurement were done at 6 sites per tooth After clinical evaluation, individuals were divided into 3 groups

1. Healthy control groups:

clinically healthy gingiva

2. Gingivitis groups:

BOP score of 10% or greater and PD≤3mm

3. Periodontitis groups:

AL ≥5 mm, PD≥6 mm and tooth loss due to periodontitis of ≤4. GCF samples were collected using strips.

Study Timeline

• Study Start: 2020-10-08
• Primary Completion: 2020-12-25
• Study Completion: 2020-12-28
• Study First Submitted: 2020-12-28
• Study First Submitted QC: 2020-12-29
• Study First Posted: 2020-12-30
• Status Verified: 2022-11
• Last Update Submitted: 2022-11-01
• Last Update Posted: 2022-11-04

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 64

Inclusion Criteria

• have periodontitis
• have 16 permanent teeth

Exclusion Criteria

• Systemic disease
• using drugs

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Periodontitis groups	gingival crevicular fluid	interdental AL ≥5 mm, PD≥6 mm
Healthy control groups	gingival crevicular fluid	clinically healthy gingiva BOP score less than 10%
Gingivitis groups	gingival crevicular fluid	BOP score of 10% or greater

Primary Outcome Measures

Name	Time	Method
Measurement of IL-6 level in GCF	GCF was taken within 1 week after clinical examination	GCF was taken interproximal region.
Measurement of IL-1Beta level	GCF was taken within 1 week after clinical examination	GCF was taken interproximal region.

Trial Locations

Locations (1)

University of Usak; 🇹🇷 Uşak, Turkey