Metabolic Profiling of Hematopoietic Stem Cells in Clonal Hematopoiesis (CHIP)

Recruiting

• Conditions: Osteoarthritis, Hip; Osteoporosis; Hip Fractures; Clonal Hematopoiesis of Indeterminate Potential; Aging
• Interventions: Other: Blood and bone marrow cell collection

• Registration Number: NCT05246813
• Lead Sponsor: Imelda Hospital, Bonheiden

Brief Summary

Bone marrow samples will be collected from patients undergoing hip arthroplasty surgery. Blood and bone marrow samples will be used for metabolic profiling and analysis of relevant CHIP mutations. Combined single-cell transcriptomics and mutation-specific single-cell genotyping (biotin-PCR using mutation-targeted primers followed by sequencing) will subsequently be performed. The gene expression profile of wildtype and mutant hematopoietic stem cells will be compared, performing both broad gene set enrichment analysis and targeted analysis of metabolic pathways.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2022-02-10
• Study First Submitted QC: 2022-02-10
• Study Start: 2022-02-11
• Study First Posted: 2022-02-18
• Status Verified: 2023-03
• Last Update Submitted: 2023-03-06
• Last Update Posted: 2023-03-07
• Primary Completion: 2024-12-31
• Study Completion: 2025-12-31

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

• Age 65 years and older

Exclusion Criteria

• known haematological condition (myelodysplasia, leukemia, cancer)
• inability to provide written informed consent

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Hip osteoarthritis patients	Blood and bone marrow cell collection	-
Osteoporotic hip fracture patients	Blood and bone marrow cell collection	-

Primary Outcome Measures

Name	Time	Method
Gene Set Enrichment Analysis (GSEA) Normalized Enrichment Score (NES)	One day

Trial Locations

Locations (1)

Imelda Hospital; 🇧🇪 Bonheiden, België, Belgium