Effect of Vegetation in Kindergartens on the Immune Response of Children
- Conditions
- Ige Responsiveness, Atopic
- Interventions
- Other: Adding of vegetation into the backyards of kindergarten
- Registration Number
- NCT02733926
- Lead Sponsor
- University of Helsinki
- Brief Summary
This study evaluates the effect of vegetation cover on certain Interleukin-10 and immunoglobulinE (IgE) among children (3-5 y old). Children will either be in a kindergarten that does not have forest floor and agricultural land in the backyards, or alternatively they will be in a kindergarten that does have forest floor and agricultural land in the backyards.
- Detailed Description
High vegetation cover is assumed to change immunoglobulinE (IgE) and interleukin-10 concentrations in blood serum. Children living in high-green kindergarten are compared with those kindergarten living utilizing backyards with a low-green cover.
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 100
- children living in study kindergartens
- immune system deficiency, e.g. HIV-infection
- a drug decreasing immune system response, e.g. oral corticosteroid
- immune system disorder that affects immune response, e.g. rheumatoid
- colitis ulcerosa, Crohn disease, diabetes
- cancer
- age less than 3 or more than 5
- born outside Finland
- intellectual disability
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description adding of vegetation into the backyards. Adding of vegetation into the backyards of kindergarten Children living in a normal city kindergarten that has plenty of vegetation in backyards. Intervention: adding of vegetation into the backyards.
- Primary Outcome Measures
Name Time Method Diversity of Gammaproteobacteria on skin. 28 days Diversity of skin Gammaproteobacteria is higher in intervention arm compared to control arm and similar compared to nature-oriented arm after 28-day intervention. It will be measured at baseline and after 28-day intervention.
- Secondary Outcome Measures
Name Time Method TGF-beta change in serum plasma 28 days An increase in TGF-beta in intervention arm but not in control arm at the end of the 28 days intervention
Skin bacterial community changes at phylum level 28 days Diversity of skin bacterial phyla, particularly Proteobacterial community, is higher in intervention arm compared to control arm. Proteobacterial community was associated with the coverage of green land cover in an earlier study. In addition, it was different in atopic and non-atopic study subjects in an earlier study. Diversity is measured as Shannon diversity index, Multivariate Homogeneity of Group Dispersions and the number of taxa (richness).
Stool bacterial community changes at phylum level 28 days Community composition of gut bacterial phyla are different between arms. Other changes include changes in relative abundance, richness and diversity.
TGF-beta: IL-17A ratio in serum plasma 28 days An increase in TGF-beta: IL17A ratio in intervention arm but not in control arm at the end of the 28 days intervention
IL-17A change in serum plasma 28 days A decrease in IL-17A in intervention arm but not in control arm at the end of the 28 days intervention
IL-10 change in serum plasma 28 days An increase in IL-10 in intervention arm but not in control arm at the end of the 28 days intervention
Skin bacterial community changes at class level 28 days Diversity of skin bacterial classes is higher in intervention arm compared to control arm. Diversity of skin bacterial classes is higher in intervention arm compared to control arm. Other community changes include changes in relative abundance, richness and in community composition. If community changes are found, the same difference is searched for in the comparison of surface soil microbiota of intervention versus standard daycare yards. Diversity is measured as Shannon diversity index, Multivariate Homogeneity of Group Dispersions and the number of taxa (richness).
Skin bacterial community changes at order level 28 days Order level diversity is higher in intervention arm than control arm (in standard daycares). Other community changes include changes in relative abundance, richness and in community composition. If community changes are found, the same difference is searched for in the comparison of surface soil microbiota of intervention versus standard daycare yards.
Skin bacterial community changes at family level are higher in intervention than control arm 28 days If differences are found, it will be checked if diversity of one or more skin bacterial families is related to soil bacterial diversity. Diversity is measured as Shannon diversity index, Multivariate Homogeneity of Group Dispersions and the number of taxa (richness).
Skin bacterial community changes at genus level 28 days Diversity of skin bacterial genera is related to arms, i.e. higher in intervention arm. If differences are found, it will be checked if these occur also in soil bacterial diversity. Diversity is measured as Shannon diversity index, Multivariate Homogeneity of Group Dispersions and the number of taxa (richness).
Associations between serum plasma TGF-beta and skin microbiota changes 28 days change in the cytokine will be separately compared with changes in bacterial diversity on skin
Stool bacterial community changes at class level 28 days Community composition of gut bacterial classes are different between arms. Other changes include changes in relative abundance, richness and diversity.
Stool bacterial community changes at order level 28 days Community composition of gut bacterial orders are different between arms. Other changes include changes in relative abundance, richness and diversity.
Associations between serum plasma IL-10 and skin Gammaproteobacterial microbiota changes 28 days change in the cytokine will be separately compared with changes in Gammaproteobacterial diversity on skin
Stool bacterial community changes at family level 28 days Community composition of gut bacterial families, particularly Ruminococcaceae, is different between arms. Ruminococcaceae contain taxa associated with the production of short chain fatty acids, such as butyrate. Other changes include changes in relative abundance, richness and diversity.
Gut Faecalibacterium community changes 28 days Community composition of gut Faecalibacterium is different between arms. Faecalibacterium has been associated with gut health and low incidence of certain immune mediated diseases.
Associations between serum plasma IL-17 and skin Gammaproteobacterial microbiota changes 28 days change in the cytokine will be separately compared with changes in Gammaproteobacterial diversity on skin
Interleukin-10 : interleukin-17A ratio in serum plasma 28 days An increase IL-10: IL-17A ratio in each arm at the end of the 28 day intervention will be examined
Trial Locations
- Locations (1)
Helsinki University
🇫🇮Lahti, Häme, Finland