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The Safety and Tolerability of Intra-abdominal t-PA and DNase on Peritonitis in Peritoneal Dialysis Patients

Phase 1
Terminated
Conditions
Peritoneal Dialysis, Continuous Ambulatory
Peritoneal Dialysis
Interventions
Drug: Tissue Plasminogen Activator (tPA)
Registration Number
NCT01478698
Lead Sponsor
Sir Charles Gairdner Hospital
Brief Summary

Hypothesis:

Intraperitoneal tPA and DNase is well tolerated at a number of different doses. Different doses of tPA and DNase will have a dose-related effect on inflammatory markers (CRP and intraperitoneal white cell count).

Aims:

1. To examine the tolerability of different doses of intraperitoneal tPA and DNase compared to standard treatment.

2. To examine the changes in biochemical and clinical outcomes of PD Peritonitis with the addition of intraperitoneal tPA and DNase to usual therapy.

Detailed Description

Not available

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
5
Inclusion Criteria
  1. PD patient
  2. Proven peritonitis (as defined as at least 2 of: (i) Symptoms and/or signs of peritonitis; (ii) Cloudy dialysate OR dialysate white cell count > 100x 106/L with >50% neutrophils; (iii) Positive culture of dialysate
  3. Age > 18 years old
Exclusion Criteria
  1. More than one organism on culture
  2. Contra-indication for systemic thrombolysis (eg. current or recent stroke, major haemorrhage or major trauma; proliferative retinopathy; major surgery in the previous 5 days)
  3. Known sensitivity to DNase or t-PA
  4. Pregnancy or lactating mother
  5. Expected survival less than 3 months
  6. Clinical indication for PD catheter removal, as defined by treating team
  7. Inability to provide written informed consent
  8. Systemic anticoagulation
  9. Severe uncontrolled hypertension
  10. Documented ulcerative gastrointestinal disease during the last three months

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Single doseTissue Plasminogen Activator (tPA)The first 5 consented participants will be administered t-PA 10mg + DNase 5mg instilled into a dialysate bag and infused for a 4 hour intraperitoneal dwell.
4 dosesrecombinant deoxyribonuclease (DNase)The next 5 consented participants will be administered tPA 10mg + DNase 5mg twice a day for 2 days.
Single doserecombinant deoxyribonuclease (DNase)The first 5 consented participants will be administered t-PA 10mg + DNase 5mg instilled into a dialysate bag and infused for a 4 hour intraperitoneal dwell.
2 dosesTissue Plasminogen Activator (tPA)The next 5 consented participants will be administered t-PA 10mg + DNase 5mg instilled twice a day for one day
2 dosesrecombinant deoxyribonuclease (DNase)The next 5 consented participants will be administered t-PA 10mg + DNase 5mg instilled twice a day for one day
4 dosesTissue Plasminogen Activator (tPA)The next 5 consented participants will be administered tPA 10mg + DNase 5mg twice a day for 2 days.
Primary Outcome Measures
NameTimeMethod
Adverse reactions72 hours

Participants will be examined for potential adverse reactions during this trial, including - pain; abnormalities in systemic coagulation profile; bleeding; and altered blood pressure.

Secondary Outcome Measures
NameTimeMethod
Biochemical markers of inflammation21 days

Biochemical markers of inflammation will be measured on days 1, 3, 5, 7, 14 and 21, including - c-reactive protein, white cell count, dialysate white cell count, procalcitonin

Clinical markers of inflammation21 days

pain score; days till pain free; proportion requiring catheter removal; days of hospitalisation; days of fever (temperature \> 37.0 degrees on at least one occasion)

Trial Locations

Locations (1)

Sir Charles Gairdner Hospital

🇦🇺

Perth, Western Australia, Australia

Sir Charles Gairdner Hospital
🇦🇺Perth, Western Australia, Australia

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