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Sequential CD19 and CD22 CAR-T Therapy for Newly Diagnosed Ph- B-ALL

Phase 1
Recruiting
Conditions
B-Cell Acute Lymphoblastic Leukemia, Adult
Interventions
Drug: CAR-T cells targeting CD19 and CD22
Registration Number
NCT04740203
Lead Sponsor
Zhejiang University
Brief Summary

Clinical Trial for the Safety and Efficacy of Sequential CD19 and CD22 CAR-T Therapy for Adult Patients With Newly Diagnosed Ph Chromosome Negative B-cell Acute Lymphoblastic Leukemia

Detailed Description

This is a prospective, single arm study. To evaluate the safety and efficacy of sequential CD19 and CD22 CAR-T cells in the treatment of adult newly diagnosed Ph chromosome negative B-cell acute lymphoblastic leukemia. The main endpoints were dose limiting toxicity (DLT) and incidence of adverse events (TEAEs).

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
50
Inclusion Criteria
  • Age≥15 years old
  • Newly diagnosed B-cell acute lymphoblastic leukemia according to the 2016 WHO classification
  • The immunophenotype of leukemia cells were CD19 and CD22 positive
  • Ph- or Ph- like negative
  • Anticipated survival time more than 12 weeks;
  • Those who voluntarily participated in this trial and provided informed consent.
Exclusion Criteria
  • History of craniocerebral trauma, conscious disturbance, epilepsy, cerebrovascular ischemia, and cerebrovascular hemorrhagic diseases;
  • Electrocardiogram shows prolonged QT interval, severe heart diseases such as severe arrhythmia in the past;
  • Pregnant (or lactating) women;
  • Patients with severe active infections (excluding simple urinary tract infection and bacterial pharyngitis);
  • Active infection of hepatitis B virus or hepatitis C virus;
  • Concurrent therapy with systemic steroids within 2 weeks prior to screening, except for the patients recently or currently receiving inhaled steroids;
  • Previously treated with any CAR-T cell product or other genetically-modified T cell therapies;
  • Creatinine>2.5mg/dl, or ALT / AST > 3 times of normal amounts, or bilirubin>2.0 mg/dl;
  • Other uncontrolled diseases that were not suitable for this trial;
  • Patients with HIV infection;
  • Any situations that the investigator believes may increase the risk of patients or interfere with the results of study.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
CAR-T therapyCAR-T cells targeting CD19 and CD22Administration of CD19 and CD22 CAR T-cells
Primary Outcome Measures
NameTimeMethod
Incidence of treatment-emergent adverse events (TEAEs)Up to 2 years after CAR-T cells infusion

Incidence of treatment-emergent adverse events \[Safety and Tolerability\]

Dose-limiting toxicity (DLT)Baseline up to 28 days after CAR-T cells infusion

Adverse events assessed according to NCI-CTCAE v5.0 criteria

Secondary Outcome Measures
NameTimeMethod
Complete Remission Rateup to 28 days after CAR-T cells infusion

Complete Remission Rate after CAR-T cell therapy

Overall survival (OS)Up to 2 years after CD19 CAR-T cells infusion

From the first infusion of CD19 CAR-T cells to death or the last visit

Leukemia-free survival (LFS)Up to 2 years after CD19 CAR-T cells infusion

From the complete remission to the occurrence of any event, including death, relapse (any one occurs first), and the last visit

Quality of lifeAt Baseline, Month 1, 3, 6, 9 and 12

Assessment using European Organisation for the Research and Treatment of Cancer Quality of Life Questionnaire Core 30 (EORTC QLQ-C30) scale \[For item1-28: max score: 112, min score: 28, higher scores mean a better outcome; for item 28-29: max score: 14, min score: 2, higher scores mean a worse outcome\] to measure Quality of life at Baseline, Month 1, 3, 6, 9 and 12

Trial Locations

Locations (1)

The First Affiliated Hospital, College of Medicine, Zhejiang University

🇨🇳

Hangzhou, Zhejiang, China

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