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A Booster Dose of Inactivated Vaccine (Vero Cell) Against EV71 in Chinese Children

Phase 2
Completed
Conditions
EV71-associated Disease
Interventions
Biological: 0/0.5ml placebo
Biological: alum-adjuvant 160U /0.5ml
Biological: adjuvant-free 640U /0.5ml
Biological: alum-adjuvant 320U /0.5ml
Biological: alum-adjuvant 640U /0.5ml
Registration Number
NCT01734408
Lead Sponsor
Jiangsu Province Centers for Disease Control and Prevention
Brief Summary

Hand, foot, and mouth disease (HFMD) is a common viral illness in infants and children caused by viruses that belong to the enterovirus genus of the picornavirus family. Although most HFMD cases do not result in serious complications, outbreaks of HFMD caused by enterovirus 71 (EV71) can present with a high rate of neurological complications, including meningoencephalitis, pulmonary complications, and can even cause infant death. HFMD caused by EV71 has become a major emerging infectious disease in Asia and the highly pathogenic potential of EV71 clearly requires the attention of world medical community.

The phase II study of inactivated vaccine (vero cell) against EV71 has completed last month in Jiangsu Province in China. The data from the phase II study suggested that the inactivated EV71 vaccine had a clinically acceptable safety and good immunogenicity for healthy Chinese children and infants. But the antibody titer against EV71 was found decreased significantly at month 8 compared to that at day 56. Considering the similar dynamic trend had also been found in the antibody against poliovirus induced by polio vaccines, and whose immunization schedule contains a booster dose administrated at 1 to 1.5 year after the first 3 doses of fundamental immunity. In order to find a better immunization schedule for children, the investigators decided to perform this booster immunity trial among these children who had received two doses of EV71 vaccines (around one year after the fundamental immunity). The investigators do the recruitment among these children who had participated in the previous phase II trial and received EV71 vaccines, and randomize them in a ratio of 2:1 to receive either a booster dose of EV71 vaccines or placebo.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
773
Inclusion Criteria
  • Had participated in the previous phase II trial and received at least 1 dose of EV71 vaccine.
  • Healthy children as established by medical history and clinical examination
  • The subjects' guardians are able to understand and sign the informed consent
  • Subjects who can and will comply with the requirements of the protocol
  • Subjects with temperature <=37.0°C on axillary setting
Exclusion Criteria
  • Had participated in the previous phase II trial and received placebo.
  • Subject who has a medical history of EV71-associated disease with specific laboratory evidence
  • <= 37 weeks gestation
  • Subjects with a birth weight <2.5 kg
  • Subject that has a medical history of any of the following: allergic history, or allergic to any ingredient of vaccine
  • Family history of seizures or progressive neurological disease
  • Family history of congenital or hereditary immunodeficiency
  • Severe malnutrition or dysgenopathy
  • Major congenital defects or serious chronic illness, including perinatal brain damage
  • Autoimmune disease
  • Bleeding disorder diagnosed by a doctor or significant bruising or bleeding difficulties with IM injections or blood draws
  • Any acute infections in last 7 days
  • Any prior administration of immunodepressant or corticosteroids in last 6month
  • Any prior administration of blood products in last 3 month
  • Any prior administration of other research medicines in last 1 month
  • Any prior administration of attenuated live vaccine in last 14 days
  • Any prior administration of subunit or inactivated vaccines in last 7 days, such as pneumococcal vaccine
  • Under the anti-TB prevention or therapy
  • Any condition that in the opinion of the investigator, may interfere with the evaluation of study objectives

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
placebo C0/0.5ml placeboA 0/0.5ml placebo in 80 children whom had received two doses of alum-adjuvant 640U/0.5ml EV71 vaccines one year before.
alum-adjuvant 160U /0.5ml EV71 vaccinealum-adjuvant 160U /0.5mlA booster dose of alum-adjuvant 160U /0.5ml EV71 vaccine in 160 children whom had received two doses of alum-adjuvant 160U/0.5ml EV71 vaccines one year before.
placebo A0/0.5ml placeboA 0/0.5ml placebo in 80 children whom had received two doses of alum-adjuvant 160U/0.5ml EV71 vaccines one year before.
adjuvant-free 640U /0.5ml EV71 vaccineadjuvant-free 640U /0.5mlA booster dose of adjuvant-free 640U /0.5ml EV71 vaccine in 160 children whom had received two doses of adjuvant-free 640U/0.5ml EV71 vaccines one year before.
alum-adjuvant 320U /0.5ml EV71 vaccinealum-adjuvant 320U /0.5mlA booster dose of alum-adjuvant 320U /0.5ml EV71 vaccine in 160 children whom had received two doses of alum-adjuvant 320U/0.5ml EV71 vaccines one year before.
placebo B0/0.5ml placeboA 0/0.5ml placebo in 80 children whom had received two doses of alum-adjuvant 320U/0.5ml EV71 vaccines one year before.
alum-adjuvant 640U /0.5ml EV71 vaccinealum-adjuvant 640U /0.5mlA booster dose of alum-adjuvant 640U /0.5ml EV71 vaccine in 160 children whom had received two doses of alum-adjuvant 640U/0.5ml EV71 vaccines one year before.
placebo D0/0.5ml placeboA 0/0.5ml placebo in 80 children whom had received two doses of adjuvant-free 640U/0.5ml EV71 vaccines one year before.
Primary Outcome Measures
NameTimeMethod
Geometric mean titer (GMT) of anti-EV71 antibodies28 days after vaccination

GMT of anti-EV71 antibodies in serum 28 days after booster dose injection

Secondary Outcome Measures
NameTimeMethod
Safety of EV71 vaccine in healthy children28 days after vaccination

Frequency of systemic and local adverse reactions in healthy children following 28 days after the boosting dose of EV71 vaccine

Seroconversion of anti-EV71 antibodies28 days after vaccination

Seroconversion of anti-EV71 antibodies in serum 28 days after vaccination

Geometric mean fold increase (GMFI) of anti-EV71 antibodies28 days after vaccination

GMFI of anti-EV71 antibodies in serum 28 days after vaccination

Trial Locations

Locations (1)

Jiangsu Provincial Center for Diseases Control and Prevention

🇨🇳

Nanjing, Jiangsu, China

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