Control of Growth and Invasiveness of Glioblastoma by Modulation of Ciliogenesis in Glioma Stem Cells. A Novel Target Against Glioblastoma for Precision Medicine. RF-2019-12368786
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Biological
研究概览
简要总结
The study aims at investigating the cilium-related transcriptome in patients-derived glioblastoma stem cells and the potential impact of modulation of cilium players in vitro, in vivo and ex vivo in glioblastoma brain organoids. Moreover, drugs inhibiting cilia disassembly will be tested. Finally, the potential prognostic role of a cilium-related gene expression signature in glioblastoma will be assessed.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •To be enrolled in the study patients must:
- •Have a radiological diagnosis of supratentorial glioblastoma, or
- •Have a radiological diagnosis of first recurrence of a primary supratentorial glioblastoma (for which a formal histopathologic diagnosis of glioblastoma had been made at first surgery), according with RANO criteria (Wen, 2010);
- •Be a candidate to neurosurgery for glioblastoma at the Operational Unit of Neurosurgery FPG;
- •Be of an age of 18 years or above;
- •Provide written informed consent for participation to the study.
排除标准
- •To be enrolled in the study patients must not:
- •Have not enough pathological material removed at surgery available both for mandatory routine histopathological diagnosis and for the present study, as judged by the Principal Investigator;
- •Have not a definitive pathological diagnosis of a primary supratentorial GBM, according with 2016 WHO classification.
研究组 & 干预措施
Biological sample collection
Collection of tumor tissue and PBMC for the generation of glioblastoma stem cell cultures and brain organoids.
干预措施: Biological sample collection (Other)
Biological sample collection
Collection of tumor tissue and PBMC for the generation of glioblastoma stem cell cultures and brain organoids.
干预措施: Dissecting ciliogenesis players (Other)
Biological sample collection
Collection of tumor tissue and PBMC for the generation of glioblastoma stem cell cultures and brain organoids.
干预措施: Validating ciliogenesis players (Diagnostic Test)
结局指标
主要结局
Biological
时间窗: Through study completion, an average of 2 years
Correlation between modulation of cilia disassembly complex genes, and tumor growth and invasion in brain organoids.
次要结局
未报告次要终点