Cytokine Profile of the Uterine Secretome After Therapeutic Endometrial Scratching

Not Applicable

Completed

• Conditions: Infertility, Female
• Interventions: Other: Pipelle de Cornier

• Registration Number: NCT03042364
• Lead Sponsor: University of Aarhus

Brief Summary

This study investigates the impact of endometrial scratching on endometrial secretion, when correlated to pregnancy.

Detailed Description

Not available

Study Timeline

• Study Start: 2016-10-01
• Study First Submitted: 2017-01-31
• Study First Submitted QC: 2017-02-01
• Study First Posted: 2017-02-03
• Status Verified: 2018-03
• Primary Completion: 2018-03-14
• Study Completion: 2018-03-14
• Last Update Submitted: 2018-03-15
• Last Update Posted: 2018-03-16

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Female
• Target Recruitment: 150

Inclusion Criteria

• IVF or ICSI patients with ≥1 previous implantation failure, despite transfer of a top quality embryo or blastocyst.
• Antagonist treatment
• FSH: 2-12 IU/L
• BMI: 18-32
• Regular menstrual cycle
• Written consent

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Exclusion Criteria

• Suspected intrauterine abnormalities Planned use of specialized media or AHA

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Intervention	Pipelle de Cornier	Endometrial scratching before standard treatment

Primary Outcome Measures

Name	Time	Method
Cytokine profile of the uterine secretome after therapeutic endometrial scratching	Within 12 months	The secretome is analyzed using a Multiplex analysis with 45 cytokines, chemokines and growth factors from ProcartaPlex Immunoassay Kit, Affymetrix, eBioscience. The samples are normalized for total protein content using a Nanodrop spectrophometer, and the protein composition is further determined by sodium dodecyl sulphate-polyacrylamide gel electropheresis (SDS-PAGE).
Immunoassay Kit:	Within 12 months	BDNF, EGF, Eotaxin, FGF-2, GMCSF, GRO-alpha, HGF, IFN alpha, IFN gamma, IL-1RA, IL-1alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL17-A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, LIF, MCP-1, MIP-1 alpha, MIP-1 beta, NGF beta, RANTES, PDGF-BB, PÅIGF-1, SCF, SDF-1 alpha, TNF alpha, TNF beta, VEGF-A, VEGF-D

Trial Locations

Locations (4)

Fertility Clinic, Herlev Hospital; 🇩🇰 Herlev, Denmark

Fertility Clinic, Skive Hospital; 🇩🇰 Skive, Denmark

Fertility Clinic,Aalborg University Hospital; 🇩🇰 Aalborg, Denmark

Fertility Clinic Horsens Hospital; 🇩🇰 Horsens, Denmark