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Clinical Trial of Intravitreal Injection of Autologous Bone Marrow Stem Cells in Patients With Retinitis Pigmentosa

Phase 1
Completed
Conditions
Retinitis Pigmentosa
Interventions
Other: Subconjunctival injection of saline
Biological: Intravitreal injection of Autologous bone marrow Stem Cell
Registration Number
NCT02280135
Lead Sponsor
Red de Terapia Celular
Brief Summary

The purpose of this study is to evaluate the safety of intravitreal injection of autologous bone marrow stem cells in patients with retinitis pigmentosa by a prospective, single-center, randomized, parallel, double-blind, phase I placebo-controlled clinical trial.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
8
Inclusion Criteria
  • Retinitis Pigmentosa bilateral diagnosis.
  • Visual acuity (measured with ETDRS) less than or equal to 20/70 and visual field below 30° central in both eyes.
  • Signed informed consent
  • Warranty sufficient adherence to protocol. It must fulfill all inclusion criteria.
Exclusion Criteria
  • Concurrence of any systemic or ocular disease that precludes or affects tracking study variables. Specifically retinal involvement with diabetes mellitus, glaucoma, macular degeneration or age.
  • Eye surgery in the previous 6 months.
  • Patients who are pregnant.
  • Patients with active lactation.
  • Physically fertile patients, defined as all women physiologically capable of becoming pregnant, including women whose career, lifestyle or sexual orientation excludes sexual intercourse with a man and women whose partners have been sterilized by vasectomy or other methods, UNLESS tHEY are using a reliable contraceptive method. This birth control method can be:
  • Complete abstinence from sexual intercourse
  • Surgical sterilization (tubal ligation)
  • Surgical sterilization partner (vasectomy)
  • Implanted or injectable hormonal contraceptives and oral.
  • Patients with cardiac disease, renal, hepatic, systemic, immune that might influence the survival of patients during the test.
  • Positive serology for hepatitis B, hepatitis C or HIV.
  • Clinical criteria or anesthetics that contraindicate the sedation or the extraction of BM (Altered coagulation system or anticoagulated patient with inability to withdraw anticoagulation, hemodynamic instability, altered skin in the puncture site, etc.)
  • Participation in other clinical trials.
  • Inability to sign informed consent or understanding.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Subconjunctival injection of salineSubconjunctival injection of salinePatients included in the trial will receive a subconjunctival injection of 0.1 ml of saline (SF) (placebo) in the fellow eye (group B or control group). In this way the patient will receive an injection in the control eye but avoid the risks of intraocular injection.
Intravitreal injection of Autologous bone marrow Stem CellIntravitreal injection of Autologous bone marrow Stem CellPatients included in the trial will receive a pars plana intravitreal injection of autologous mononuclear cells (MNC) of bone marrow (BM) in one eye (experimental group A or group). The eye in which autologous BM MNCs were injected will be determined randomly. The average dose will be 30 million of cells (5-60 million) diluted in 0.1 ml. of saline.
Primary Outcome Measures
NameTimeMethod
Rate of serious and non-serious adverse events related with the use of bone marrow mononuclear cells in patients with retinitis pigmentosa12 months from baseline
Secondary Outcome Measures
NameTimeMethod
Visual acuity (VA): Test ETDRS (Early Treatment Diabetic Retinopathy Study).12 months from baseline
Study eye fundus: Made by Retinography and Angiography fluorescein.12 months from baseline
Visual evoked potentials with Pattern Reversal (VEP) (altered / no altered).12 months from baseline
Quality of Life: Questionnaire VFQ-25 (Visual Function Questionnaire-25).12 months from baseline
Color Vision: Ishihara Color Test.12 months from baseline
Electrical retinal function: electroretinogram (ERG) (altered / unaltered).12 months from baseline
Contrast sensitivity: CSV-1000E.12 months from baseline
Width of retinal macula layer and nerve fiber: Measured with Optical Coherence Tomography Spectral domain (OCT)(Topcon 3D OCT-2000 Spectral Domain OCT).12 months from baseline
Visual field (VF) and macular sensitivity (The Humphrey perimeter).12 months from baseline
Intraocular pressure (IOP): measured in mm Hg with applanation tonometer Haag Streit AT 900.12 months from baseline
Examination of the anterior and posterior pole: Made with biomicroscopy (BMC).12 months from baseline

Trial Locations

Locations (1)

Clinical Universitary Hospital Virgen de la Arrixaca

🇪🇸

El Palmar, Murcia, Spain

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