(NOD)-Like Receptors CARD Domain-Containing 4 Levels in Individuals With Periodontitis
- Conditions
- PeriodontitisGingivitisHealthy
- Interventions
- Other: SalivaOther: gingival crevicular fluid
- Registration Number
- NCT04850768
- Lead Sponsor
- Saglik Bilimleri Universitesi
- Brief Summary
NLRC4, an inflammasome , is a cytosolic multiprotein complex involved in the initiation and modulation of the immune response. Periodontitis is a chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. It has been said that proinflammatory cytokines released in the microenvironment of periodontitis can increase the expression of NLRC4 inflammasome genes. The authors think that NLRC4 may play a role in the periodontitis. The aim of this study is to compare the NLRC4, IL-1β and IL-10 levels of healthy and periodontitis individuals.
- Detailed Description
Periodontitis is a destructive chronic inflammatory disease that can start with localized inflammatory reactions created by the supporting tissues surrounding the teeth against microorganisms and then result in loss of teeth. Inflammasomes are cytosolic multiprotein complex involved in the initiation and modulation of the immune response.
Interleukine -1β ( IL-1β ) levels are associated with periodontal destruction in the pathogenesis of periodontal disease. Interleukine-10 (IL-10) has been reported to decrease periodontal disease progression rate.
Gingival crevicular fluid shows the cellular response in the periodontium. The most important host-related ingredients are inflammatory markers, including cytokines, enzymes, and interleukins. Saliva analysis is used in the diagnosis of systemic conditions as well as in the diagnosis of oral pathologies.
Both NLRC4 and NLRP3 inflammasomes have been shown to play an important role in the induction of interleukin-1β secretion. Periodontitis is induced by a complex microbial biofilm containing multiple Gram-positive and Gram-negative bacteria (including flagellate microorganisms) capable of activating a large number of NLRs. It has been said that proinflammatory cytokines released in the microenvironment of periodontitis can increase the expression of NLRC4 inflammasome genes. Evidence on the role of inflammasomes in periodontal disease is still very poor. The aim of this study is to compare the NLRC4, IL-1β and IL-10 levels of healthy and periodontitis individuals.
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 75
- systemically healthy individuals who does not have a disease under treatment, individuals without regular systemic medication
- smoking receiving periodontal treatment in the last 6 months pregnancy menopausal or menstrual period
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Gingivitis Saliva Individuals with Gingival Inflammation Periodontitis Saliva Individuals with Periodontitis Periodontitis gingival crevicular fluid Individuals with Periodontitis Gingivitis gingival crevicular fluid Individuals with Gingival Inflammation Healthy gingival crevicular fluid Individuals with Periodontally Healthy Healthy Saliva Individuals with Periodontally Healthy
- Primary Outcome Measures
Name Time Method IL-10 levels through study completion, an average of 6 months saliva and DOS levels
IL-1β levels through study completion, an average of 6 months saliva and DOS levels
NLRC4 through study completion, an average of 6 months saliva and DOS levels
- Secondary Outcome Measures
Name Time Method Plaque Index up to 20 weeks Silness-Löe
Bleeding on Probe up to 20 weeks Ainamo\&Bay
Probing Depth up to 20 weeks millimeter
Trial Locations
- Locations (1)
Saglik Bilimleri Universitesi
🇹🇷Istanbul, Turkey