Tissue Resident Memory T Cells in Early & Late Cases of Generalized Non-segmental Vitiligo
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Vitiligo
- Sponsor
- Kasr El Aini Hospital
- Enrollment
- 29
- Locations
- 1
- Primary Endpoint
- Double CD8+ and CD69+ immunofluorescence
- Last Updated
- 4 years ago
Overview
Brief Summary
This study aims to detect the presence and diversity of Tissue resident memory T cell populations in early and late cases of generalized non-segmental Vitiligo.
Detailed Description
* Written informed consent obtained from every participant. * Detailed history taking including onset, course, duration of the disease, aggravating factors and received medications * VASI score assessed in every patient. * Patients photographed, divided into two groups early \& late cases and 3 mm punch biopsies will be taken from perilesional and non lesional skin. * Biopsies processed and stained for CD8 \& CD69 in cases of early group, \& late group and controls by immunofluorescence.
Investigators
Rana F Hilal, MD
Assistant Professor
Kasr El Aini Hospital
Eligibility Criteria
Inclusion Criteria
- •- Patients with Generalized non-segmental Vitiligo ( lesions in more than one anatomical site ).
- •Both genders.
- •Early group: onset less than or equal 6 months.
- •Late group: onset more than 6 months
Exclusion Criteria
- •Localized or Segmental Vitiligo.
- •Age \< 14 years.
- •Pregnant females
- •Patient who received treatment of any type within the last 3 months for the late group.
- •Patients with other skin diseases or immunosuppression
Outcomes
Primary Outcomes
Double CD8+ and CD69+ immunofluorescence
Time Frame: 6months-1 year
Number of Tissue resident Memory cells by double stained by CD8+ \&CD 69+ in early and late generalized non segmental vitiligo patients
CD69+ immunofluorescence
Time Frame: 6months-1 year
Number of Tissue resident Memory cells by positively stained by CD8+ in early and late generalized non segmental vitiligo patients
CD8+ immunofluorescence
Time Frame: 6months-1 year
Number of Tissue resident Memory cells by positively stained by CD8+ in early and late generalized non segmental vitiligo patients
VASI
Time Frame: 6months-1 year
Vitiligo Area and Severity index minimum 0 maximum 100, the larger the number the worse the condition