Resident Memory T Cells in Vitiligo

• Conditions: Vitiligo

• Registration Number: NCT05223738
• Lead Sponsor: Kasr El Aini Hospital

Brief Summary

This study aims to detect the presence and diversity of Tissue resident memory T cell populations in early and late cases of generalized non-segmental Vitiligo.

Detailed Description

* Written informed consent obtained from every participant.

* Detailed history taking including onset, course, duration of the disease, aggravating factors and received medications

* VASI score assessed in every patient.

* Patients photographed, divided into two groups early \& late cases and 3 mm punch biopsies will be taken from perilesional and non lesional skin.

* Biopsies processed and stained for CD8 \& CD69 in cases of early group, \& late group and controls by immunofluorescence.

Study Timeline

• Study Start: 2020-12-31
• Primary Completion: 2021-03-30
• Status Verified: 2022-01
• Study First Submitted: 2022-01-25
• Study First Submitted QC: 2022-01-25
• Last Update Submitted: 2022-01-25
• Study Completion: 2022-02-01
• Study First Posted: 2022-02-04
• Last Update Posted: 2022-02-04

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 29

Inclusion Criteria

• • Patients with Generalized non-segmental Vitiligo ( lesions in more than one anatomical site ).
• Both genders.
• Early group: onset less than or equal 6 months.
• Late group: onset more than 6 months

Exclusion Criteria

• Localized or Segmental Vitiligo.
• Age < 14 years.
• Pregnant females
• Patient who received treatment of any type within the last 3 months for the late group.
• Patients with other skin diseases or immunosuppression

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Double CD8+ and CD69+ immunofluorescence	6months-1 year	Number of Tissue resident Memory cells by double stained by CD8+ \&CD 69+ in early and late generalized non segmental vitiligo patients
CD69+ immunofluorescence	6months-1 year	Number of Tissue resident Memory cells by positively stained by CD8+ in early and late generalized non segmental vitiligo patients
CD8+ immunofluorescence	6months-1 year	Number of Tissue resident Memory cells by positively stained by CD8+ in early and late generalized non segmental vitiligo patients
VASI	6months-1 year	Vitiligo Area and Severity index minimum 0 maximum 100, the larger the number the worse the condition

Trial Locations

Locations (1)

Kasr El Ainy University hospital; 🇪🇬 Cairo, Egypt