Staphylokinase and ABO Group Phenotype: New Players in Staphylococcus Aureus Implant-associated Infections Development
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Nantes University Hospital
- Enrollment
- 88
- Locations
- 1
- Primary Endpoint
- IAI and NC nasal carriage isolates genetic background
Study Overview
Brief Summary
The purpose of this study is to identify bacterial and/or clinical features involved in the pathogenesis of Staphylococcus aureus implant-associated infections (IAI). Materials & methods: In total, 57 IAI S. aureus and 31 nasal carriage (NC) S. aureus isolates were studied. Staphylococcus aureus genetic background was obtained by microarray analysis. Multilocus sequence typing was performed to determine clonal complexes (CC). Biofilm production was investigated by resazurin and crystal violet methods
Study Design
- Study Type
- Observational
- Observational Model
- Case Control
- Time Perspective
- Retrospective
Eligibility Criteria
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •infected patients with S. Aureus IAI infection
- •noninfected patients screened for S. aureus nasal colonization at the orthopedic consultation ward
Exclusion Criteria
- Not provided
Arms & Interventions
control group
patients with Staphylococcus aureus nasal carriage
Intervention: Staphylococcus aureus nasal carriage infection (Other)
IAI patients group
patients with Staphylococcus aureus of implant-associated infections
Intervention: IAI infection (Other)
Outcomes
Primary Outcomes
IAI and NC nasal carriage isolates genetic background
Time Frame: september 2012
IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied
biofilm production measurement in the two groups
Time Frame: september 2012
The primary aim of this study is to identify phenotypic (biofilm production) markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery. By using resazurin method, IAI and NC nasal carriage isolates were divided into three groups. Isolates were classified as strong, moderate and weak biofilm producers.
Clonal complexes distribution
Time Frame: september 2012
The primary aim of this study is to identify genotypic markers in two different groups of S. aureus strains isolated from patients with IAI or screened from nasal carrier in noninfected patients undergoing orthopedic surgery. IAI and NC isolates genetic background was determined in order to compare the prevalence of individual virulence factor genes. All genes provided by the Alere StaphyType DNA microarray were studied.
Secondary Outcomes
- biofilm production correlation with genotype(december 2013)
- Association of ABO group phenotype and IAI S. aureus genotype(december 2013)
- Correlation between Clonal Complexes distribution , genotype and clinical parameters(december 2013)