Study of the Impact of Freezing-thawing Procedures and the Prolonged Culture of Embryos on Epigenetic Regulation in Humans
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 298
- 试验地点
- 2
- 主要终点
- Analysis of the expression of several genes subjected to imprinting (H19, IGF2, IGF2R, MEST, PEG3, SNRPN, IGF2, KCNQ1, GRB10 and PLAGL1)
研究概览
简要总结
For this project, 4 groups of 38 infants have been created so as to evaluate the impact of Assisted-Reproduction Technology (ART) and more particularly the two procedures classically used in ART: the freezing/thawing of embryos and prolonged embryo culture (five days of culture in appropriate in vitro conditions to allow development of the embryo) in contrast with shorter culture of only two days.
Participation consists in accepting samples to be taken at the birth of the infant:
- from the umbilical cord.
- from the placenta.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Patients who have provided written informed consent,
- •Patients with national health insurance cover,
- •Maternal age at conception between 20 and 43 years,
- •Paternal age at conception between 18 and 50 years,
- •Singleton pregnancy
排除标准
- •--Maternal disease:
- •pulmonary, cardiac
- •renal and metabolic diseases (including type 1 and 2 diabetes prior to the pregnancy)
- •systemic inflammatory diseases
- •hypertension
- •neurological diseases
- •chronic hepatitis B or C
- •HIV infection
结局指标
主要结局
Analysis of the expression of several genes subjected to imprinting (H19, IGF2, IGF2R, MEST, PEG3, SNRPN, IGF2, KCNQ1, GRB10 and PLAGL1)
时间窗: Baselines
DNA / RNA extraction
Level of methylation and the level of expression of Genomic Imprinting (GI) and Transposable Elements (TE)
时间窗: Baselines
DNA / RNA extraction
Analysis of the expression of 3 transposable elements (HERV-K, LINE-1 and ALU)
时间窗: Baselines
DNA / RNA extraction
次要结局
未报告次要终点