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Phenotypic characterization by impression cytology of the corneal surface reconstructed by simple limbal epithelial transplantatio

Not Applicable
Recruiting
Conditions
imbal stem cell deficiency after SLET
Limbal stem cell deficiency
LSCD
Simple limbal epithelium transplantation
SLET
Limbal stem cells
Stem cells
Cornea
Registration Number
TCTR20160310002
Lead Sponsor
Faculty of Medicine Siriraj Hospital, Mahidol University
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Recruiting
Sex
All
Target Recruitment
10
Inclusion Criteria

1. Patients with unilateral or bilateral limbal stem cell deficiency (LSCD) from various causes including Stevens-Johnson syndrome, chemical or thermal burns, chronic keratitis, multiple corneal surgeries, and prolonged use of eye drops
2. Patients having experienced auto-SLET or allo-SLET
3. Patients who used to have been diagnosed total LSCD in the SLET-treated eye
4. Patients who have been diagnosed LSCD with impression cytology before receiving SLET

Exclusion Criteria

1.Patients aged below 18 years
2. Patients who used to have been diagnosed partial LSCD in the SLET-treated eye
3. Patients having received penetrating keratoplasty following SLET
4. HIV-infected patients
5. Patients with ongoing eye infection
6. Patients with uncontrolled glaucoma
7. Patients with pregnancy
8. Patients unable to follow-up
9. Patients with non-compliance, physically or mentally

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Characteristics of impression cytology of cornea after SLET Three time points of 3-month interval, all post-operative Impression cytology and immunofluorescence for specific markers of corneal and conjunctival epitheli
Secondary Outcome Measures
NameTimeMethod
Correlation of impression cytology with in vivo confocal microscopy and clinical outcome Three time points of 3-month intervall, all post-operative Impression cytology, slit-lamp examination and in vivo confocal microscopy
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