Effects of Procyanidine on Semen Parameters and DNA Fragmentation Index During Cryopreservation of Abnormal Human Semen Samples: a Prospective, Double-blind Trial
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 50
- 试验地点
- 1
- 主要终点
- Progressive sperm motility
研究概览
简要总结
Cryopreservation is the storage of biological material at subzero temperatures at which biochemical processes of cell metabolism and the biochemical reactions that lead to cell death are slowed, interrupted or stopped.Many investigators have focused on the use of antioxidants in the freezing media to reduce the negative effects of reactive oxygen species(ROS) on spermatozoa and in this context addition of vitamin E to cryoprotective medium enhanced the post-thaw sperm motility and preserved sperm DNA integrity , superoxide dismutase (SOD) and catalase (CAT) were added to boar spermatozoa freezing media and they not only increased sperm motility and viability but also decreased post-thaw ROS generation which led to improvement in results of in vitro fertilizing with thawed spermatozoa.However, the impact of in vitro addition of proanthocyanidins to human semen before cryopreservation on post-thawing semen parameters, DFI has not been studied yet. The research question evaluated in the current study was whether semen samples of infertile men supplemented or not with procyanidine before cryopreservation, differ in post-thawing semen parameters and sperm DNA fragmentation index (DFI).
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •Infertility defined as:
- •At least twelve (12) months of non-achieving pregnancy despite unprotected sexual intercourse or six (6) months, if the female is > 35 years of age AND
- •At least two (2) semen analyses with at least one abnormal parameter (sperm concentration, motility and morphology), according to WHO 2010 criteria.
- •Not on any type of infertility treatment for the last three (3) months
- •Sperm concentration > 8 x 106/ml and semen volume at least 2.5 ml for technical reasons.
- •Normal hormonal profile (TSH, FSH, LH, total testosterone, prolactin)
- •Seminal white blood cells < 1 x 106/ml
- •No abnormalities in scrotal ultrasound.
排除标准
- •Underlying genetic cause of infertility
- •History of undescended testis (cryptorchidism)
- •History of orchidectomy
- •History of testicular cancer
- •History of severe cardiac, hepatic or renal disease.
- •History of endocrine disease (primary or secondary hypogonadism, hyperprolactinemia, thyroid disease, pituitary or adrenal disease)
- •History of epididymo-orchitis, prostatitis, genital trauma, testicular torsion, inguinal or genital surgery
- •History of systemic disease or treatment during the last three (3) months
- •Positive sperm culture for Chlamydia or Ureaplasma urealyticum
- •Female infertility factors
- •Body mass index (BMI) > 30 kg/m2
- •Participation in another interventional study and a likelihood of being unavailable for follow-up.
研究组 & 干预措施
procyanidine group
干预措施: procyanidine (Dietary Supplement)
Control group
结局指标
主要结局
Progressive sperm motility
时间窗: 3 Hours after ejaculation and immediately after thawing with or without Procyanidine
percentage (%) of decrease
次要结局
- Sperm vitality(3 Hours after ejaculation and immediately after thawing with or without Procyanidine)
- Sperm DNA fragmentation index(3 Hours after ejaculation and immediately after thawing with or without Procyanidine)
- Sperm morphology(3 Hours after ejaculation and immediately after thawing with or without Procyanidine)
研究者
E.M. Kolibianakis
Associate Professor
Aristotle University Of Thessaloniki